Description:

·         The antigens of Toxocara sp. are separated by gel electrophoresis, and then transferred to the nitrocellulose membrane (i.e. to the respective WB strips).

·         If specific antibodies are present in the sample, they will bind to the respective antigens (Mr = 120 kDa, 32 kDa and 26 kDa).

·         The complex is labelled with Conjugate and detected through a colour reaction with substrate (BCIP/NBT).

·         The kit enables 16 tests.

·         High sensitivity and specificity.

·         Total assay time: approximately 3 h.

·         For test validity verification, the strips are equipped with a control band (which indicates the kit functionality and sensitivity).

·         Positive and Negative controls included enabling validation of the tests.

·         Identical (i.e. interchangeable) reagents (except for Conjugate) in all BLOT kits.

·         All reagents supplied are ready to use.

·         The kit may be used sequentially for smaller batches of samples, reagents are provided in sufficient quantities.

·         Evaluation of results – the intensity of the bands can be evaluated visually or by means of software (Immunoblot Software).

Applications:

·         Laboratory test for the detection of toxocara infection.