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BLOT Toxoplasma IgG

BLOT Toxoplasma IgG
EAN Code: 8595635300707
Catalog number: TgGB16
Package size: 16 tests
Producer: TestLine Clinical Diagnostics s.r.o.

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Contact person:

Name: Ing. Michal Šteffl
Phone: +420 549 121 259
E-mail: trade@testlinecd.com

Western Blot kit for the detection of IgG antibodies to the respective antigens of Toxoplasma gondii in human serum or plasma.

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Contact person:

Name: Ing. Michal Šteffl
Phone: +420 549 121 259
E-mail: trade@testlinecd.com

Description

Description:

·         The antigens of Toxoplasma gondii are separated by gel electrophoresis, and then transferred to the nitrocellulose membrane (i.e. to the respective WB strips).

·         If specific antibodies are present in the sample, they will bind to the respective antigens Mr = 30 kDa.

·         The complex is labelled with Conjugate and detected through a colour reaction with substrate (BCIP/NBT).

·         The kit enables 16 tests.

·         High sensitivity and specificity.

·         Total assay time: approximately 3 h.

·        For test validity verification, the strips are equipped with a control band (which indicates the kit functionality and sensitivity).

·         Positive and Negative controls included enabling validation of the tests.

·         Identical (i.e. interchangeable) reagents (except for Conjugate) in all BLOT kits.

·         All reagents supplied are ready to use.

·         The kit may be used sequentially for smaller batches of samples, reagents are provided in sufficient quantities.

·         Evaluation of results – the intensity of the bands can be evaluated visually or by means of software (Immunoblot Software).

Applications:

·         Laboratory test for the detection of infection with Toxoplasma gondii in humans, confirmatory test to ELISA.

Brief assay procedure:

1.       Dilute controls and tested samples (1:188,5).

2.       Pipette diluted controls and samples into the channels of the tray and insert the WB strips.

3.       Incubate for 60 minutes at room temperature using a shaking apparatus.

4.       Wash 3 times for 5 minutes.

5.       Add Conjugate.

6.       Incubate for 60 minutes at room temperature using a shaking apparatus.

7.       Wash 3 times for 5 minutes.

8.       Add substrate (BCIP/NBT).

9.       Incubate for 10 minutes at room temperature using a shaking apparatus.

10.   Wash 2 times for 5 minutes in distilled water.

11.   Dry the strips and evaluate results using the validation strip enclosed.

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Have a question? Need a help?

Ing. Michal Šteffl

 

Phone: +420 549 121 259

E-mail: trade@testlinecd.com