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EIA Bordetella parapertussis IgG

EIA Bordetella parapertussis IgG
EAN Code: 8595635305061
Catalog number: BppG96
Package size: 96 tests
Regulatory status: IVD CE
Storage: 2-8 °C
Producer: TestLine Clinical Diagnostics s.r.o.

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Contact person:

Name: MUDr. Jana Dočkalová
Phone: +420 549 121 205
E-mail: trade@testlinecd.com

The immunoenzymatic assay is intended for the diagnosis of Bordetella parapertussis infection using IgG antibodies in human serum or plasma in the general population. The semi-quantitative manual assay is designed for professional use in a laboratory.

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Description

Description:

·         Microtitre wells are coated with a combination of selected parts antigens specific for Bordetella parapertussis.

·         If specific antibodies are present, they bind to the antigen, are labeled by the Conjugate in the following steps and are detected by color reaction with a single component substrate (TMB-Complete).

·         The kit allows 96 tests, including controls in a split microtiter plate with color-coded strips and breakable wells.

Advantages:

·         The total assay time is about 2 hours.

·         High sensitivity and specificity of the test.

·         Kit includes CUT-OFF, Positive Control and Negative Control.

·         Semi-quantitative evaluation in the Index of Positivity (IP).

·         Ready-to-use, color-coded components.

·         Single-component substrate.

·         Interchangeable components with the exception of kit specific components (Controls, Conjugate, Plate).

Application:

·         Serological determination of antibodies to Bordetella parapertussis.

·         Diagnostics of post-infection and post-vaccination antibody response.

Brief assay procedure:

1.       Dilute samples (1:101).

2.       Pipette Controls and diluted Samples.

3.       Incubate at 37°C for 30 min.

4.       Aspirate and wash the wells 5×

5.       Pipette Conjugate.

6.       Incubate at 37°C for 30 min.

7.       Aspirate and wash the wells 5×

8.       Pipette Substrate (TMB-Complete).

9.       Incubate at 37°C for 30 min.

10.   Pipette Stop Solution.

11.   Read color intensity at 450 nm.

12.   Evaluate the test.

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