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EIA Helicobacter MONO IgA

EIA Helicobacter MONO IgA
EAN Code: 8595635302800
Catalog number: HMA096
Package size: 96 tests
Producer: TestLine Clinical Diagnostics s.r.o.

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Contact person:

Name: Ing. Michal Šteffl
Phone: +420 549 121 259
E-mail: trade@testlinecd.com

Enzyme immunoassay for the determination of IgA antibodies to Helicobacter pylori (monospecies) in human serum or plasma.

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Contact person:

Name: Ing. Michal Šteffl
Phone: +420 549 121 259
E-mail: trade@testlinecd.com

Description

Description:

·         Microtitre wells are coated with the clinical significant H. pylori strain with proteins CagA (120 kDa) and VacA (87 kDa).

·         If present, specific antibodies bind to the antigen. The complex is labelled with Conjugate and detected through a colour reaction with substrate (TMB-Complete).

·         The kits enables 96 quantitative tests (including controls and calibrators) within the microtitre plate made of colour-coded strips and breakable wells.

·         High sensitivity and specificity.

·         Total assay time: approximately 1.5 h.

·         CUT-OFF included.

·         Calibrators (10, 20, 80, 320 AU/ml) included.

·         Quantitative evaluation in AU/ml units

·         Sample diluent, TMB-Complete and Avidity Solution are interchangeable if provided with identical numeric marking (i.e. Sample Diluent 2, Sample Diluent 3, etc.).  Stop Solution and Wash Solution are interchangeable in all TestLine kits.

·         Controls, Conjugate and substrate (TMB-Complete) are supplied in working strength.

·         Colour reagents for easy pipetting.

Applications:

·         Screening test for the detection of infection with H. pylori.

·         Monitoring of antibody levels after eradication of H. pylori.

Brief assay procedure:

1.       Dilute controls and tested samples (1:101).

2.       Pipette diluted controls and samples.

3.       Incubate for 30 min at 37 °C.

4.       Aspirate and wash the wells 5 times.

5.       Add Conjugate.

6.       Incubate for 30 min at 37 °C.

7.       Aspirate and wash the wells 5 times.

8.       Add substrate (TMB-Complete).

9.       Incubate for 15 min at 37 °C.

10.   Add Stopping solution (H2SO4).

11.   Read photometrically at 450 nm.

12.   Evaluate results.

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Have a question? Need a help?

Ing. Michal Šteffl

 

Phone: +420 549 121 259

E-mail: trade@testlinecd.com