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EIA SS-A/Ro60

EIA SS-A/Ro60
EAN Code: 8595635304019
Catalog number: Ro6096
Package size: 96 tests
Producer: TestLine Clinical Diagnostics s.r.o.

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Contact person:

Name: Ing. Michal Šteffl
Phone: +420 549 121 259
E-mail: trade@testlinecd.com

Enzyme immunoassay for the determination of IgG antibodies to Sm antigen in human serum or plasma.

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Contact person:

Name: Ing. Michal Šteffl
Phone: +420 549 121 259
E-mail: trade@testlinecd.com

Description

Description:

·         Microtitre wells are coated with mixture of recombinant SmB antigen and native SmD antigen.

·         If present, specific antibodies bind to the antigen. The complex is labelled with Conjugate and detected through a colour reaction with substrate (TMB-Complete).

·         The kit enables 96 quantitative tests (including controls and calibrators) ) within the microtitre plate made of colour-coded strips and breakable wells.

·         Total assay time: approximately 1.5 h.

·         High sensitivity and specificity.

·         Colour reagents for easy pipetting.

·         Semiquantitative evaluation using the index of positivity (IP) or quantitative evaluation in U/ml units.

·         Controls, Conjugate and substrate (TMB-Complete) are supplied in working strength.

Applications:

·         Test for detection of antibodies to respective ENA antigens.

·         Differential diagnostics of systemic autoimmune diseases.

Brief assay procedure:

1.       Dilute tested samples (1:101).

2.       Pipette controls, calibrators and diluted samples.

3.       Incubate for 30 min at 37 °C.

4.       Aspirate and wash the wells 5 times.

5.       Add Conjugate.

6.       Incubate for 30 min at 37 °C.

7.       Aspirate and wash the wells 5 times.

8.       Add substrate (TMB-Complete).

9.       Incubate for 15 min at 37 °C.

10.   Add Stopping solution (H2SO4).

11.   Read photometrically at 450 nm.

12.   Evaluate results.

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Have a question? Need a help?

Ing. Michal Šteffl

 

Phone: +420 549 121 259

E-mail: trade@testlinecd.com