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EIA Toxoplasma IgG

EIA Toxoplasma IgG
EAN Code: 8595635303067
Catalog number: TgG096
Package size: 96 tests
Producer: TestLine Clinical Diagnostics s.r.o.

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Contact person:

Name: Ing. Michal Šteffl
Phone: +420 549 121 259
E-mail: trade@testlinecd.com

Enzyme immunoassay for the detection of IgG antibodies to Toxoplasma gondii and their avidity in human serum or plasma.

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Contact person:

Name: Ing. Michal Šteffl
Phone: +420 549 121 259
E-mail: trade@testlinecd.com

Description

Description:

·         Microtitre wells are coated with the antigen of Toxoplasma gondii.

·         If present, specific antibodies bind to the antigen. The complex is labelled with Conjugate and detected through a colour reaction with substrate (TMB-Complete).

·         The kit enables 96 tests (including controls) within the microtitre plate made of colour-coded strips and breakable wells.

·         The kit contains the Avidity solution, which enables quantitative determination of the binding strength of an antibody-antigen complex.

·         High sensitivity and specificity.

·         Total assay time: approximately 2.5 h.

·         Controls, Conjugate and substrate (TMB-Complete) are supplied in working strength.

·         Sample diluent, TMB-Complete and Avidity Solution are interchangeable if provided with identical numeric marking (i.e. Sample Diluent 2, Sample CUT-OFF included.

·         Calibrators (6, 60, 240 IU/ml) included.

·         Semiquantitative evaluation using the index of positivity (IP) or quantitative evaluation in international units IU/ml.

·         Colour reagents for easy pipetting.

Applications:

·         Screening test for the detection of infection with Toxoplasma gondii in humans.

·         Checking of therapy results using the semiquantitative or quantitative determination.

·         Differential diagnosis of acute and chronic phase of the disease using the Avidity test.

Brief assay procedure:

1.       Dilute samples (1:101).

2.       Pipette controls and diluted samples.

3.       Incubate for 60 min at 37 °C.

4.       Aspirate and wash the wells 5 times.

5.       Add Conjugate.

6.       Incubate for 60 min at 37 °C.

7.       Aspirate and wash the wells 5 times.

8.       Add substrate (TMB-Complete).

9.       Incubate for 20 min at 37 °C.

10.   Add Stopping solution (H2SO4).

11.   Read photometrically at 450 nm.

12.   Evaluate results.

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Have a question? Need a help?

Ing. Michal Šteffl

 

Phone: +420 549 121 259

E-mail: trade@testlinecd.com