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SmartEIA Adenovirus IgM

SmartEIA Adenovirus IgM
Catalog number: SK-ILE-ADV03
Package size: 96 tests
Producer: Immunolab GmbH

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Contact person:

Name: Ing. Michal Šteffl
Phone: +420 549 121 259
E-mail: trade@testlinecd.com

ELISA kit for the detection and quantification of human IgM antibodies to Adenovirus in human serum or plasma. The SmartEIA kits are specifically designed for fully automated processing using the Agility instrument.

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Contact person:

Name: Ing. Michal Šteffl
Phone: +420 549 121 259
E-mail: trade@testlinecd.com

Description

Description:

·         Microtitre wells are coated with the antigen.

·         If present, specific antibodies bind to the antigen. The complex is labelled with Conjugate and detected through a color reaction with substrate (TMB-Complete).

·         The kit enables 96 tests (including controls and calibrators) within the microtitre plate made of breakable wells.

Brief assay procedure:

1.       Dilute samples (1:101).

2.       Pipette controls, calibrators and diluted samples.

3.       Incubate for 60 min at 37 °C.

4.       Aspirate and wash the wells 4 times.

5.       Add Conjugate.

6.       Incubate for 30 min at 37 °C

7.       Aspirate and wash the wells 5 times.

8.       Add substrate (TMB-Complete).

9.       Incubate for 20 min at 37 °C

10.   Add Stopping solution (H2SO4).

11.   Read photometrically at 450 nm.

12.   Evaluate results.

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Have a question? Need a help?

Ing. Michal Šteffl

 

Phone: +420 549 121 259

E-mail: trade@testlinecd.com