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SmartEIA ENA screen plus

SmartEIA ENA screen plus
EAN Code: 8595635306082
Catalog number: SK-ENA096
Package size: 96 tests
Producer: TestLine Clinical Diagnostics s.r.o.

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Contact person:

Name: Ing. Michal Šteffl
Phone: +420 549 121 259
E-mail: trade@testlinecd.com

Enzyme immunoassay for the detection of IgG antibodies to extractable nuclear antigens and centromere B in human serum or plasma. SmartEIA kit is specifically designed for automated analysis using the Agility instrument.

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Contact person:

Name: Ing. Michal Šteffl
Phone: +420 549 121 259
E-mail: trade@testlinecd.com

Description

Description:

·         Microtitre wells are coated with extractable nuclear antigens (SS-A/Ro, SS-B/La, Scl 70, Sm, Sm/RNP, Jo-1) and centromere B (CEN-B) as antigen.

·         If present, specific antibodies bind to the antigen. The complex is labelled with Conjugate and detected through a colour reaction with substrate (TMB-Complete).

·         The kit enables 96 tests (including controls) within the microtitre plate made of colour-coded strips and breakable wells.

·         CUT-OFF included.

·         Total assay time: approximately 1.5 hour.

·         High sensitivity and specificity.

·         Semiquantitative evaluation using the index of positivity (IP).

·         Controls, Conjugate and substrate (TMB-Complete) are supplied in working strength.

·         Colour reagents for easy pipetting.

Application:

·         Screening test for early diagnostics on suspicion of the system autoimmune disorder.

Brief assay procedure:

1.       Dilute samples (1:101).

2.       Pipette controls, calibrators and diluted samples.

3.       Incubate for 30 min at 37 °C.

4.       Aspirate and wash the wells 5 times.

5.       Add Conjugate.

6.       Incubate for 30 min at 37 °C.

7.       Aspirate and wash the wells 5 times.

8.       Add substrate (TMB-Complete).

9.       Incubate 15 min at 37 °C.

10.   Add Stopping solution (H2SO4).

11.   Read photometrically at 450 nm.

12.   Evaluate results.

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Have a question? Need a help?

Ing. Michal Šteffl

 

Phone: +420 549 121 259

E-mail: trade@testlinecd.com