Menu Search My Account
Registration

Login

Registration Restore password
Cart Empty cart
cz en

SmartEIA Gliadin IgA

SmartEIA Gliadin IgA
EAN Code: 8595635306532
Catalog number: SK-GlA096
Package size: 96 tests
Producer: TestLine Clinical Diagnostics s.r.o.

Sample request

Sample ordering is possible only for registered users

If you are already registered, please log in
If you are new customer register

Contact person:

Name: Ing. Michal Šteffl
Phone: +420 549 121 259
E-mail: trade@testlinecd.com

Enzyme immunoassay for the detection of IgA antibodies to gliadin in human serum or plasma. SmartEIA kit is specifically designed for automated analysis using the Agility instrument.

If you are already registered, please login

Request Product Export to PDF Print

Have a question? Need a help?

Do not hesitate and call us or fill in a query form and we will contact you soon.

Contact person:

Name: Ing. Michal Šteffl
Phone: +420 549 121 259
E-mail: trade@testlinecd.com

Description

Description:

·         Microtitre wells are coated with gliadin and relating cereal proteins.

·         If present, specific antibodies bind to the antigen. The complex is labelled with Conjugate and detected through a colour reaction with substrate (TMB-Complete).

·         The kit enables 96 tests (including controls) within the microtitre plate made of colour-coded strips and breakable wells.

·         High sensitivity and specificity.

·         Total assay time: approximately 1.5 hour

·         CUT-OFF included.

·         Semiquantitative evaluation using the index of positivity (IP).

·         Sample diluent, TMB-Complete and Avidity Solution are interchangeable if provided with identical numeric marking (i.e. Sample Diluent 2, Sample Diluent 3, etc.).  Stop Solution and Wash Solution are interchangeable in all TestLine kits.

·         Colour reagents for easy pipetting.

Applications:

·         Screening test for the detection of sensitivity to gluten in humans.

·         Monitoring of gluten free diet effect.

Brief assay procedure:

1.       Dilute samples (1:101).

2.       Pipette, controls and diluted samples.

3.       Incubate for 30 min at 37 °C.

4.       Aspirate and wash the wells 5 times.

5.       Add Conjugate.

6.       Incubate for 30 min at 37 °C.

7.       Aspirate and wash the wells 5 times.

8.       Add substrate (TMB-Complete).

9.       Incubate 15 min at 37 °C.

10.   Add Stopping solution (H2SO4).

11.   Read photometrically at 450 nm.

12.   Evaluate results.

Download

Have a question? Need a help?

Ing. Michal Šteffl

 

Phone: +420 549 121 259

E-mail: trade@testlinecd.com