Description:

·         Microtitre wells are coated with the antigen.

·         If present, specific antibodies bind to the antigen. The complex is labelled with Conjugate and detected through a color reaction with substrate (TMB-Complete).

·         The kit enables 96 tests (including controls and calibrators) within the microtitre plate made of breakable wells.

Brief assay procedure:

1.       Dilute samples (1:101).

2.       Pipette controls, calibrators and diluted samples.

3.       Incubate for 60 min at 37 °C.

4.       Aspirate and wash the wells 4 times.

5.       Add Conjugate.

6.       Incubate for 30 min at 37 °C

7.       Aspirate and wash the wells 5 times.

8.       Add substrate (TMB-Complete).

9.       Incubate for 20 min at 37 °C

10.   Add Stopping solution (H2SO4).

11.   Read photometrically at 450 nm.

12.   Evaluate results.