Description:
· Microtitre wells are coated with the antigen.
· If present, specific antibodies bind to the antigen. The complex is labelled with Conjugate and detected through a color reaction with substrate (TMB-Complete).
· The kit enables 96 tests (including controls and calibrators) within the microtitre plate made of breakable wells.
Brief assay procedure:
1. Dilute samples (1:101).
2. Pipette controls, calibrators and diluted samples.
3. Incubate for 60 min at 37 °C.
4. Aspirate and wash the wells 4 times.
5. Add Conjugate.
6. Incubate for 30 min at 37 °C
7. Aspirate and wash the wells 5 times.
8. Add substrate (TMB-Complete).
9. Incubate for 20 min at 37 °C
10. Add Stopping solution (H2SO4).
11. Read photometrically at 450 nm.
12. Evaluate results.