Description:

·         Microtitre wells are coated with highly purified human thyreoglobulin as antigen.

·         If present, specific antibodies bind to the antigen. The complex is labelled with Conjugate and detected through a colour reaction with substrate (TMB-Complete).

·         The kits enables 96 tests (including controls and calibrators) within the microtitre plate made of colour-coded strips and breakable wells.

·         Total assay time: approximately 1.5 h.

·         High sensitivity and specificity.

·         Colour reagents for easy pipetting.

·         Semiquantitative evaluation using the index of positivity (IP) or quantitative evaluation in international units IU/l.

·         Controls, Conjugate and substrate (TMB-Complete) supplied in working strength.

Application:

·         Laboratory diagnostics of autoimmune diseases of thyroid gland.

Brief assay procedure:

1.       Dilute tested samples (1:101).

2.       Pipette controls, calibrators and diluted samples.

3.       Incubate for 30 min at 37 °C.

4.       Aspirate and wash the wells 5 times.

5.       Add Conjugate.

6.       Incubate for 30 min at 37 °C.

7.       Aspirate and wash the wells 5 times.

8.       Add substrate (TMB-Complete).

9.       Incubate for 15 min at 37 °C.

10.   Add Stopping solution (H2SO4).

11.   Read photometrically at 450 nm.

12.   Evaluate results.