Description:

·         Microtitre wells are coated with the excretory-secretory ('ES') antigen isolated from the cultured larvae of Toxocara canis.

·         If present, specific antibodies bind to the antigen. The complex is labelled with Conjugate and detected through a colour reaction with substrate (TMB-Complete).

·         The kit enables 96 tests (including controls) within the microtitre plate made of colour-coded strips and breakable wells.

·         High sensitivity and specificity.

·         Total assay time: approximately 1.5 h.

·         CUT-OFF included.

·         Semiquantitative evaluation using the index of positivity (IP).

·         Controls, Conjugate and substrate (TMB-Complete) are supplied in working strength.

·         Colour reagents for easy pipetting.

·         Each batch is tested in the National Reference Laboratory.

Applications:

·         Laboratory test for the detection of toxocariasis (larva migrans).

·         Monitoring of antibody levels following therapy.

Brief assay procedure:

1.       Dilute samples (1:101).

2.       Pipette controls and diluted samples.

3.       Incubate for 30 min at 37 °C.

4.       Aspirate and wash the wells 5 times.

5.       Add Conjugate.

6.       Incubate for 30 min at 37 °C.

7.       Aspirate and wash the wells 5 times.

8.       Add substrate (TMB-Complete).

9.       Incubate for 15 min at 37 °C.

10.   Add Stopping solution (H2SO4).

11.   Read photometrically at 450 nm.

12.   Evaluate results.