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SmartEIA VZV IgM

SmartEIA VZV IgM
EAN Code: 8595635306136
Catalog number: SK-VZVM96
Package size: 96 tests
Producer: TestLine Clinical Diagnostics s.r.o.

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Contact person:

Name: Ing. Michal Šteffl
Phone: +420 549 121 259
E-mail: trade@testlinecd.com

Enzyme immunoassay for the detection of IgM antibodies to Varicella zoster virus (VZV) in human serum, plasma or cerebrospinal fluid. SmartEIA kit is specifically designed for automated analysis using the Agility instrument.

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Contact person:

Name: Ing. Michal Šteffl
Phone: +420 549 121 259
E-mail: trade@testlinecd.com

Description

Description:

·         Microtitre wells are coated with the purified and inactivated VZV antigen with high content of specific immuno dominant epitopes.

·         If present, specific antibodies bind to the antigen. The complex is labelled with Conjugate and detected through a colour reaction with substrate (TMB-Complete).

·         The kit enables 96 tests (including controls) within the microtitre plate made of colour-coded strips and breakable wells.

·         Total assay time: approximately 1.5 h.

·         High sensitivity and specificity.

·         Colour reagents for easy pipetting.

·         CUT-OFF included.

·         Semiquantitative evaluation using the index of positivity (IP).

·         Reagents are supplied in working strength.

·         Sample diluent, TMB-Complete and Avidity Solution are interchangeable if provided with identical numeric marking (i.e. Sample Diluent 2, Sample Diluent 3, etc.).  Stop Solution and Wash Solution are interchangeable in all TestLine kits.

·         Shelf life: 15 months.

Applications:

·         Screening test for the detection of specific IgM antibodies in human serum or plasma.

·         Checking of therapy results using the semiquantitative detection.

·         Disease phase diagnostics.

Brief assay procedure:

1.       Dilute samples of serum/plasma (1:101), cerebrospinal fluid (1:3).

2.       Pipette controls and diluted samples.

3.       Incubate for 30 min at 37 °C.

4.       Aspirate and wash wells 5 times.

5.       Add Conjugate.

6.       Incubate for 30 min at 37 °C.

7.       Aspirate and wash wells 5 times.

8.       Add substrate (TMB-Complete).

9.       Incubate for 30 min at 37 °C. 

10.   Add Stopping solution.

11.   Read photometrically at 450 nm.

12.   Evaluate results.

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Have a question? Need a help?

Ing. Michal Šteffl

 

Phone: +420 549 121 259

E-mail: trade@testlinecd.com