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Varicella Zoster virus DNA Quantification Kit (in25)

Varicella Zoster virus DNA Quantification Kit (in25)
Catalog number: GT-VZVin25
Package size: 25 tests
Producer: genetrac s.r.o.

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Contact person:

Name: Ing. Michal Šteffl
Phone: +420 549 121 259
E-mail: trade@testlinecd.com

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Contact person:

Name: Ing. Michal Šteffl
Phone: +420 549 121 259
E-mail: trade@testlinecd.com

Description

Description:

·         Diagnostic kit for qualitative and quantitative detection of DNA of Varicella Zoster Virus utilizing amplification of ORF62 gene by real-time polymerase chain reaction (PCR) and measurement of the increase of concentration of the specific product using fluorescence probe.

·         The kit includes diluted calibrators allowing for quantitative evaluation of the results.

·         MasterMix contains DNA nucleotide dUTP and enzyme uracil-DNA-glycosylase (UDG) which enables removal of potential contamination from simultaneous side reactions.

·         MasterMix contains the internalized standard which allows to monitor correct reaction process.

·         The kit uses “hot start” technology in order to minimize non-specific reactions and provide maximum sensitivity of detection.

·         The integrated system of controls allows to monitor the reaction.

·         Unique composition of the components ensures great stability of the kit, the GC clips are used.

·         The kit is validated on various types of thermocyclers.

·         User friendly assay procedure.

·         Package size: 25 tests.

·         Shelf life: 12 months.

Brief assay procedure:

1.       Dissolve and homogenize MasterMix and pipette it to PCR tubes.

2.       Add DNA isolate.

3.       Add calibrators.

4.       Close PCR tubes, centrifuge shortly all drops from wall of the tubes.

5.       Put the tubes into thermocycler and perform the amplification.

6.       Evaluate qualitative and quantitative results.

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Have a question? Need a help?

Ing. Michal Šteffl

 

Phone: +420 549 121 259

E-mail: trade@testlinecd.com