Description:

·         Microtitre wells are coated with native, recombinant or mixture of antigens.

·         If present, specific antibodies bind to the antigen. The complex is labelled with Conjugate and detected through a colour reaction with substrate (TMB-Complete).

·         The kit enables 12 tests within the microtitre plate.

·         Total assay time: approximately 1.5 h.

·         Colour-coded strips and breakable wells.

·         Sample diluent, TMB-Complete and Avidity Solution are interchangeable if provided with identical numeric marking (i.e. Sample Diluent 2, Sample Diluent 3, etc.). Stop Solution and Wash Solution are interchangeable in all TestLine kits.

·         CUT-OFF included.

·         Colour and ready-to-use reagents.

·         Semiquantitative evaluation using the index of positivity (IP).

Applications:

·         Confirmatory test to EIA ENA screen plus.

·         Differential diagnosis of systemic autoimmune diseases by determination of specific ENAs.

Brief assay procedure:

·         Dilute samples: serum/plasma 1:101 (10 μl + 1 ml).

·         Pipette Controls and diluted samples – 100 μl.

·         Incubate at 37°C for 30 min.

·         Aspirate and wash the wells 5×.

·         Pipette Conjugate – 100 μl.

·         Incubate at 37°C for 30 min.

·         Aspirate and wash the wells 5×.

·         Pipette Substrate (TMB-Complete) – 100 μl.

·         Incubate at 37°C for 15 min.

·         Pipette Stop Solution – 100 μl.

·         Read colour intensity at 450 nm/620 nm.

·         Evaluate the results.