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EIA Yersinia enterocolitica IgA

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EIA Yersinia enterocolitica IgA
EAN Code: 8595635308161
Catalog number: YA0096
Package size: 96 tests
Producer: TestLine Clinical Diagnostics s.r.o.
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Contact person:

Name: Leona Kopečková
Phone: +420 549 121 259
E-mail: sales@testlinecd.com

Enzyme immunoassay for the detection of IgA antibodies to Yersinia enterocolotica in human serum or plasma.

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Description

Description:

·         A mixture of highly specific recombinant Yersinia enterocolitica antigens is bound to the well surface of the microtiter plate.

·         If specific antibodies are present, they bind to the antigen, labelled Conjugate in the following steps and are detected by color reaction with a single component substrate (TMB-Complete).

·         The kit allows 96 tests, including controls in a split microtiter plate with color-coded strips and breakable wells.

·         The total assay time is about 1.5 hours.

·         Kit includes CUT-OFF.

·         Semi-quantitative evaluation in the Index of Positivity (IP).

·         High sensitivity and specificity of the test.

·         Color solutions make work easier and reduce test error.

·         Possibility of gradual processing of the kit, the solutions are in sufficient excess.

·         Kit contains solutions in working dilution and single component substrate (TMB-Complete).

·         Sample Diluent, TMB-Complete and Avidity Solution are interchangeable in TestLine EIA kits if they have the same numerical indication. Stop and Wash Solution are universal.

 

Application:

·         Detection of specific antibodies against Yersinia enterocolitica.

·         Confirmation of post-infectious antibodies.

·         Diagnostics of the disease phase by a significant increase or decrease of antibodies.

 

Brief assay procedure:

1.       Dilute samples (1:101)

2.       Pipette Controls and diluted Samples

3.       Incubate at 37°C for 30 min

4.       Aspirate and wash the wells 5×

5.       Pipette Conjugate

6.       Incubate at 37°C for 30 min

7.       Aspirate and wash the wells 5×

8.       Pipette Substrate (TMB-Complete)

9.       Including blank

10.   Incubate at 37°C for 30 min

11.   Pipette Stop Solution

12.   Read colour intensity at 450 nm

13.   Evaluate the test

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Have a question? Need a help?

Leona Kopečková

 

Phone: +420 549 121 259

E-mail: sales@testlinecd.com