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Microblot-Array ANA 45

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Microblot-Array ANA 45
EAN Code: 8595635307706
Catalog number: ANA45MA96
Package size: 96 tests
Producer: TestLine Clinical Diagnostics s.r.o.
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Contact person:

Name: Ing. Michal Šteffl
Phone: +420 549 121 259
E-mail: sales@testlinecd.com

Microblot-Array kit for the detection of specific IgG antibodies to nuclear antigens in human serum or plasma.

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Description

Description

·         Used antigen:

o   Group 1 (ANA): Jo-1, PL-7, PL-12, EJ, OJ, KS, YARS, ZoA, ZoB, HMGCR, SAE-1, SAE-2, SRP54, Mi-2, TIF1γ, MDA5, NXP2, NT5CN1A, PmScl 75, PmScl 100, CENP A, CENP B, Scl70, POLR3A, NOR90, PDGFR-β, Fibrillarin, Th/To, Ro52, Ro60, La, SmB, SmD, RNP A, RNP C, RNP 68/70, P0, Ku, Nucleolin, dsDNA, Histone, Nucleosome, PCNA; Supplementary antigens: M2, DFS70

o   Group 2 (Myositis): Jo-1, PL-7, PL-12, EJ, OJ, KS, YARS, ZoA, ZoB, HMGCR, SAE-1, SAE-2, SRP54, Mi-2, TIF1γ, MDA5, NXP2, NT5CN1A; Supplementary antigens: Ro52, PmScl 75, PmScl 100, Ku

o   Group 3 (Scleroderma): CENP A, CENP B, Scl70, POLR3A, NOR90, PDGFR-β, Fibrillarin, Th/To, PmScl 75, PmScl 100, RNP A, RNP C, RNP 68/70; Supplementary antigens: Ro52, Ku, M2

o   Group 4 (SLE and other connective tissue diseases): dsDNA, Histone, Nucleosome, PCNA, SmB, SmD, RNP A, RNP C, RNP 68/70, P0, Ku, Nucleolin, Ro52, Ro60, La, NOR90

·         Antigens are applied in the form of microdots (spots) in triplets to a nitrocellulose membrane in a microtiter well format

·         If specific antibodies are present in the sample, they will bind to the respective separate antigens

·         The complex is labelled with Conjugate and detected through a colour reaction with Substrate (BCIP/NBT)

·         The kit enables 96 tests

·         Short incubation periods, total assay time: approximately 1.5 h.

·         Each individual test includes control spots, which serves to check functionality and sensitivity of the sets, and also the spots for checking the presence of the conjugate

·         Included positive controls enable validation of the tests

·         For quantitative evaluation the wells contain calibration spots

·         All reagents are supplied ready to use

·         The kit may be used sequentially for smaller batches of samples (reagents are provided in sufficient quantities)

·         Evaluation using Microblot-Array reader and the appropriate software

 

Applications

·         Confirmation for ELIA or other screening tests

·         Identification of individual antibodies types, differentiation of individual systemic autoimmune diseases

 

Brief assay procedure

·         Dilute samples of serum/plasma (1:51)

·         Pipette diluted samples

·         Incubate at room temperature for 30 min.

·         Wash 3 times for 5 min.

·         Add Conjugate

·         Incubate at room temperature for 30 min.

·         Wash 3 times for 5 min.

·         Add Substrate (BCIP/NPT)

·         Incubate at room temperature for 15 min.

·         Wash 2 times for 5 min. in distilled water

·         Dry and evaluate test

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Have a question? Need a help?

Ing. Michal Šteffl

 

Phone: +420 549 121 259

E-mail: sales@testlinecd.com