· A mixture of highly specific recombinant Yersinia enterocolitica antigens is bound to the well surface of the microtiter plate.
· If specific antibodies are present, they bind to the antigen, labeled Conjugate in the following steps and are detected by color reaction with a single component substrate (TMB-Complete).
· The kit allows 96 tests, including controls in a split microtiter plate with color-coded strips and breakable wells.
· The total assay time is about 1.5 hours.
· Kit includes CUT-OFF.
· Semi-quantitative evaluation in the Index of Positivity (IP).
· High sensitivity and specificity of the test.
· Color solutions make work easier and reduce test error.
· Possibility of gradual processing of the kit, the solutions are in sufficient excess.
· Kit contains solutions in working dilution and single component substrate (TMB-Complete).
· Sample Diluent, TMB-Complete and Avidity Solution are interchangeable in TestLine EIA kits if they have the same numerical indication. Stop and Wash Solution are universal.
· Detection of specific antibodies against Yersinia enterocolitica.
· Confirmation of post-infectious antibodies.
· Diagnostics of the disease phase by a significant increase or decrease of antibodies.
Brief assay procedure:
1. Dilute samples (1:101)
2. Pipette Controls and Calibrators
3. Incubate at 37°C for 30 min
4. Aspirate and wash the wells 5×
5. Pipette Conjugate
6. Incubate at 37°C for 30 min
7. Aspirate and wash the wells 5×
8. Pipette Substrate (TMB-Complete)
9. Including blank
10. Incubate at 37°C for 30 min
11. Pipette Stop Solution
12. Read colour intensity at 450 nm
13. Evaluate the test