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TA bulk Immunocomplex

TA bulk Immunocomplex
EAN Code: 8595635303517
Catalog number: CIKb00
Package size: 1500 tests
Producer: TestLine Clinical Diagnostics s.r.o.

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Contact person:

Name: Ing. Michal Šteffl
Phone: +420 549 121 259
E-mail: sales@testlinecd.com

Turbidimetric assay for the detection of circulating immunocomplexes in human serum.

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Description

Description:

·         "Selective" precipitation of soluble human serum antigen-antibody immunocomplexes in polyethylenglycol (PEG) and borate buffer.

·         Precipitate amount is measured turbidimetrically at 450 nm.

·         The kit enables 1500 determinations using biochemical analyzers.

·         Total assay time: approximately 1.5 hour.

·         Negative control, CUT-OFF and Positive control included.

·         Evaluation using the index of positivity (IP) or in units: (Absorbance of sample in PEG buffer - Absorbance of sample in borate buffer) x 1000.

·         Shelf life: 24 months.

Application:

·         Detection of immunocomplexes generated in later stages of disease.

·         Monitoring of disease process by observation of dynamics of circulating immunocomplexes concentration dynamics (e.g. rheumatoid arthritis, SLE, chronic glomerulonephritis, tumors - late stages, recovery of VHB ).

Brief assay procedure:

1.       Prepare controls, incubate them for 30 min at room temperature.

2.       Dilute samples.

3.       Pipette borate buffer and PEG buffer.

4.       Add controls and diluted samples.

5.       Mix thoroughly.

6.       Incubate for 60 min at 21-25 °C.

7.       Mix thoroughly.

8.       Read photometrically at 450 nm.

9.       Evaluate results.

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Have a question? Need a help?

Ing. Michal Šteffl

 

Phone: +420 549 121 259

E-mail: sales@testlinecd.com