Description:
- Microtitre wells are coated with native, recombinant or mixture of antigens.
- If specific antibodies are present, they bind to the antigen, are labeled by the Conjugate in the following steps and are detected by color reaction with a single component substrate (TMB-Complete).
- The kit allows 96 tests, including controls in a split microtiter plate with color-coded strips and breakable wells.
Advantages:
- The total assay time is about 2 hours.
- High sensitivity and specificity of the test.
- Kit includes CUT-OFF, Positive Control and Negative Control, Calibrators.
- Semi-quantitative evaluation in the Index of Positivity (IP).
- Ready-to-use, color-coded components.
- Single-component substrate.
- Interchangeable components with the exception of kit specific components (Controls, Conjugate, Plate).
Application:
- Confirmatory test to EIA ENA screen plus.
- Differential diagnosis of systemic autoimmune diseases by determination of specific ENAs.
Brief assay procedure:
- Dilute samples (1:101).
- Pipette Controls and diluted Samples.
- Incubate at 37°C for 30 min.
- Aspirate and wash the wells 5×
- Pipette Conjugate.
- Incubate at 37°C for 30 min.
- Aspirate and wash the wells 5×
- Pipette Substrate (TMB-Complete).
- Incubate at 37°C for 15 min.
- Pipette Stop Solution.
- Read color intensity at 450 nm.
- Evaluate the test.
SmartEIA kits are specifically designed for automated processing on the Agility® instrument, Dynex Technologies, Inc.
