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EAN Code: 8595635307034
Catalog number: ANC3L20
Package size: 20 tests
Regulatory status: IVD CE
Storage: 2-8 °C
Producer: TestLine Clinical Diagnostics s.r.o.

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Contact person:

Name: Ing. Zuzana Gavačová
Phone: +420 549 121 273

The immunoblot assay is intended for the diagnosis of systemic autoimmune diseases using IgG antibodies to neutrophil cytoplasm and GBM in human serum or plasma in the general population. The qualitative and semi-quantitative manual assay is designed for professional use in a laboratory.

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·         Antigen used: MPO, PR3, GBM.

·         Recombinant antigens are applied to the nitrocellulose membrane (i.e. to the respective BLOTLINE (BL) strips).

·         If specific antibodies are present in the sample, they will bind to the respective antigens.

·         The complex is labelled with Conjugate and detected through a colour reaction with substrate (BCIP/NBT).

·         The kit enables 20 tests.

·         Short incubation periods, total assay time: approximately 1.5 h.

·         High sensitivity and specificity.

·         All reagents supplied are ready to use.

·         The kit may be used sequentially for smaller batches of samples (reagents are provided in sufficient quantities).

·         BL strips equipped with conjugate control band and a control band indicating kits functionality and sensitivity.

·         Positive and Negative controls included enabling validation of the tests.

·         Evaluation of results – the intensity of the bands can be evaluated visually or by means of software (Immunoblot Software).


·         Laboratory test for the detection of antineutrophil cytoplasmic antibodies, confirmatory test to ELISA.

Brief assay procedure:

1.       Dilute serum/plasma samples (1:51)

2.       Pipette diluted samples and controls into the channels of the tray and insert the BL strips.

3.       Incubate for 30 min at room temperature using a shaker.

4.       Wash 3 times for 5 min.

5.       Add Conjugate.

6.       Incubate for 30 min at room temperature using a shaker.

7.       Wash 3 times for 5 min.

8.       Add substrate (BCIP/NBT).

9.       Incubate for 15 min at room temperature using a shaker.

10.   Wash twice for 5 min in distilled water.

11.   Dry the strips and evaluate results using the validation strip enclosed or using Immunoblot Software.


Have a question?
Need a help?

Ing. Zuzana Gavačová

+420 549 121 273