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BLOT Toxocara IgG

BLOT Toxocara IgG
EAN Code: 8595635300684
Catalog number: TcGB16
Package size: 16 tests
Regulatory status: IVD CE
Producer: TestLine Clinical Diagnostics s.r.o.

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Contact person:

Name: Mgr. Miroslav Herczeg
Phone: +420 549 121 273
E-mail: herczeg@testlinecd.com

The immunoblot assay is intended for the diagnosis of Toxocara spp. infection using IgG antibodies in human serum or plasma in the general population. The qualitative and semi-quantitative manual assay is designed for professional use in a laboratory.

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Description

Description:

·         The antigens of Toxocara sp. are separated by gel electrophoresis, and then transferred to the nitrocellulose membrane (i.e. to the respective WB strips).

·         If specific antibodies are present in the sample, they will bind to the respective antigens (Mr = 120 kDa, 32 kDa and 26 kDa).

·         The complex is labelled with Conjugate and detected through a colour reaction with substrate (BCIP/NBT).

·         The kit enables 16 tests.

·         High sensitivity and specificity.

·         Total assay time: approximately 3 h.

·         For test validity verification, the strips are equipped with a control band (which indicates the kit functionality and sensitivity).

·         Positive and Negative controls included enabling validation of the tests.

·         Identical (i.e. interchangeable) reagents (except for Conjugate) in all BLOT kits.

·         All reagents supplied are ready to use.

·         The kit may be used sequentially for smaller batches of samples, reagents are provided in sufficient quantities.

·         Evaluation of results – the intensity of the bands can be evaluated visually or by means of software (Immunoblot Software).

Applications:

·         Laboratory test for the detection of toxocara infection.

Brief assay procedure:

1.       Dilute controls and tested samples (1:188,5).

2.       Pipette diluted controls and samples into the channels of the tray and insert the WB strips.

3.       Incubate for 60 minutes at room temperature using a shaking apparatus.

4.       Wash 3 times for 5 minutes.

5.       Add Conjugate.

6.       Incubate for 60 minutes at room temperature using a shaking apparatus.

7.       Wash 3 times for 5 minutes.

8.       Add substrate (BCIP/NBT).

9.       Incubate for 10 minutes at room temperature using a shaking apparatus.

10.   Wash 2 times for 5 minutes in distilled water.

11.   Dry the strips and evaluate results using the validation strip enclosed.

 

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Have a question?
Need a help?

Mgr. Miroslav Herczeg

+420 549 121 273 herczeg@testlinecd.com