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BlueBLOT-LINE EBV IgG

BlueBLOT-LINE EBV IgG
EAN Code: 8595635308239
Catalog number: BD-EBGL24
Package size: 24 tests
Regulatory status: IVD CE
Storage: 2-8 °C
Producer: TestLine Clinical Diagnostics s.r.o.

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Contact person:

Name: MUDr. Jana Dočkalová
Phone: +420 549 121 205
E-mail: trade@testlinecd.com

The immunoblot assay is intended for the diagnosis of EBV infection using IgG antibodies to recombinant antigens in human serum or plasma in the general population. The semi-quantitative automated assay is designed for professional use in a laboratory.

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Description

Description:

·         Each individual test contains all ready-to-use reagents required to perform the test.

·         Used antigens: a combination of selected portions of specific antigens (EBNA-1, VCA-P18, VCA-P23, EA-D).

·         Individual recombinant antigens are transfered to the nitrocellulose membrane; in the presence of specific antibodies in the sample, they bind to respective antigens. The complex is labelled with Conjugate and detected through a colour reaction with substrate.

·         Short incubation times, total examination time about 1.25 hours.

·         Evaluation using Immunoblot Software (TestLine).

Application

·         Diagnostics of Epstein-Barr infection.

·         Confirmation test of ELISA.

Brief assay procedure:

1.      Insert testing strips and cartridges into the BlueDiver Instrument.

2.      Pipette samples.

3.      Fully automated incubation and washing steps in BlueDiver Instrument

4.      Incubation 30 minutes.

5.      Wash 3 x 2 min.

6.      Conjugate dispensing.

7.      Incubation 20 min.

8.      Wash 3 x 2 min.

9.      Substrate dispensing.

10.   Incubation 10 min.

11.   Wash 1x 2 min.

12.   Evaluation of test results using the BlueScan scanner and Immunoblot Software.

 

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