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EAN Code: 8595635302596
Catalog number: EBL480
Package size: 480 tests
Producer: TestLine Clinical Diagnostics s.r.o.

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Contact person:

Name: MVDr. Ivo Hofmann, CSc.
Phone: +420 549 121 209

Modular enzyme immunoassay for the detection of antibodies to Enzootic Bovine Leukosis Virus (EBLV) in individual and pooled bovine serum samples.

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·         2 versions of the kit (screening, confirmative).

·         Microtitre wells are coated with the virus antigen (p-24) using monoclonal antibodies (screening microtitre plate is coated with the EBLV antigen).

·         The proportion of screening and confirmative microplates can be changed on request.

·         Screening kit (5 microplates – 12 × 8 wells) for up to 480 screening tests for samples of blood serum, including controls.

·         The whole kit may be used sequentially for smaller batches of samples.

·         The positive control corresponds to the Danish positive reference serum E4 (diluted 10 times).

·         Total time of survey according to sample incubation duration:

-          over night: results in second day.

-          rapid test: approximately 2.5 h.

·         The evaluation: comparing the absorbance of the sample against the absorbance of the negative control.

·         Tested by the Veterinary Research Institute, Brno, Czech Republic and the State Veterinary Institutes in the Czech Republic.

·         Approved by the Institute for State Control of Veterinary Biologicals and Medicaments, Czech Republic.

·         The kit is manufactured in accordance with Good Manufacturing Practice (GMP).

·         Shelf life: 12 months.


·         Screening of EBL occurrence in a cattle population.

·         Control of current sanitation process in cattle herds.

·         Ongoing screening in herds after sanitation.

·         Control of transported and quarantined animals (export, import).

Brief assay procedure:

1.       Dilute samples and controls (1:20) in microplate wells.

2.       Incubate:

a)      over night: 14–18 hours at 4–8 °C

b)      rapid test: 60 min at 37 °C

3.       Aspirate and wash the wells 4 times.

4.       Add Conjugate.

5.       Incubate for 60 min at 37 °C.

6.       Aspirate and wash the wells 4 times.

7.       Add substrate (TMB-Complete).

8.       Incubate for 10 min at room temperature.

9.       Add Stopping solution (H2SO4).

10.   Read the absorbance photometrically at 450 nm.

11.   Evaluate results.


Have a question? Need a help?

MVDr. Ivo Hofmann, CSc.


Phone: +420 549 121 209