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EIA Bordetella pertussis Toxin IgA

EIA Bordetella pertussis Toxin IgA
EAN Code: 8595635301209
Catalog number: BpAT96
Package size: 96 tests
Regulatory status: IVD CE
Storage: 2-8 °C
Producer: TestLine Clinical Diagnostics s.r.o.

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Contact person:

Name: MUDr. Jana Dočkalová
Phone: +420 549 121 205

The immunoenzymatic assay is intended for the diagnosis of Bordetella pertussis infection using IgA antibodies in human serum or plasma in the general population. The quantitative manual assay is designed for professional use in a laboratory.

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·         Microtitre wells are coated with highly purified toxin of Bordetella pertussis.

·         If specific antibodies are present, they bind to the antigen, are labeled by the Conjugate in the following steps and are detected by color reaction with a single component substrate (TMB-Complete).

·         The kit allows 96 tests, including controls in a split microtiter plate with color-coded strips and breakable wells.


·         The total assay time is about 2 hours.

·         High sensitivity and specificity of the test.

·         Kit includes CUT-OFF, Positive Control, Negative Control and Calibrators (1, 10, 20, 70 IU/ml).

·         Semi-quantitative evaluation in the Index of Positivity (IP) or quantitative evaluation in AU/ml.

·         Ready-to-use, color-coded components.

·         Single-component substrate.

·         Interchangeable components with the exception of kit specific components (Controls, Conjugate, Plate).


·         Diagnostics of post-infection and post-vaccination antibody response.

·         Serological determination of antibodies to pertussis toxin.

Brief assay procedure:

1.       Dilute samples (1:101).

2.       Pipette Controls and diluted Samples.

3.       Incubate at 37°C for 30 min.

4.       Aspirate and wash the wells 5×

5.       Pipette Conjugate.

6.       Incubate at 37°C for 30 min.

7.       Aspirate and wash the wells 5×

8.       Pipette Substrate (TMB-Complete).

9.       Incubate at 37°C for 30 min.

10.   Pipette Stop Solution.

11.   Read color intensity at 450 nm.

12.   Evaluate the test.