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EIA CMV IgM

EIA CMV IgM
EAN Code: 8595635302695
Catalog number: CMM096
Package size: 96 tests
Regulatory status: IVD CE2265
Storage: 2-8 °C
Producer: TestLine Clinical Diagnostics s.r.o.

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Contact person:

Name: MUDr. Jana Dočkalová
Phone: +420 549 121 205
E-mail: trade@testlinecd.com

The immunoenzymatic assay is intended for the diagnosis of CMV infection using IgM antibodies in human serum, plasma or cerebrospinal fluid in the general population. The semi-quantitative manual assay is designed for professional use in a laboratory.

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Description

Description:

  • Microtitre wells are coated with the purified and inactivated antigen isolated from AD169 strain of Cytomegalovirus.
  • If specific antibodies are present, they bind to the antigen, are labeled by the Conjugate in the following steps and are detected by color reaction with a single component substrate (TMB-Complete).
  • The kit allows 96 tests, including controls in a split microtiter plate with color-coded strips and breakable wells.

Advantages:

 

  • The total assay time is about 1 hour 30 minutes.
  • High sensitivity and specificity of the test.
  • Kit includes CUT-OFF, Positive Control and Negative Control, Calibrators
  • Semi-quantitative evaluation in the Index of Positivity (IP)
  • Sample diluent contains IgG/RF sorbent.
  • Ready-to-use, color-coded components
  • Single-component substrate
  • Interchangeable components with the exception of kit specific components (Controls, Conjugate, Plate)
  • Sample diluent contains IgG/RF sorbent.

Application:

  • Screening test for the detection of specific IgM antibodies in human serum or plasma.
  • Checking of therapy results using the semiquantitative detection.
  • Disease phase diagnostics.

Brief assay procedure:

  1. Dilute samples (1:101).
  2. Pipette Controls and diluted Samples.
  3. Incubate at 37°C for 30 min.
  4. Aspirate and wash the wells 5×.
  5. Pipette Conjugate.
  6. Incubate at 37°C for 30 min.
  7. Aspirate and wash the wells 5×.
  8. Pipette Substrate (TMB-Complete).
  9. Incubate at 37°C for 15 min.
  10. Pipette Stop Solution.
  11. Read color intensity at 450 nm
  12. Evaluate the test.
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