Description:

·         Microtitre wells are coated with recombinant Coxsackievirus B1-B5 antigen.

·         If specific antibodies are present, they bind to the antigen, are labeled by the Conjugate in the following steps and are detected by color reaction with a single component substrate (TMB-Complete).

·         The kit allows 96 tests, including controls in a split microtiter plate with color-coded strips and breakable wells.

Advantages:

·         Kit includes CUT-OFF, Positive Control, Negative Control and Calibrators (5, 20, 80, 320 U/ml).

·         Single-component substrate.

·         Ready-to-use, color-coded components.

·         Interchangeable components with the exception of kit specific components (Controls, Conjugate, Plate).

·         The total assay time is about 1,5 hours.

·         Semi-quantitative evaluation in the Index of Positivity (IP) or quantitative evaluation in U/ml.

·         High sensitivity and specificity of the test.

Application:

·         Screening test for the detection of specific IgG antibodies in human serum or plasma.

·         Differential detection of Enteroviruses in the sample.

Brief assay procedure:

1.      Dilute samples (1:101).

2.      Pipette Controls and diluted Samples.

3.      Incubate at 37°C for 30 min.

4.      Aspirate and wash the wells 5×

5.      Pipette Conjugate.

6.      Incubate at 37°C for 15 min.

7.      Aspirate and wash the wells 5×

8.      Pipette Substrate (TMB-Complete).

9.      Incubate at 37°C for 15 min.

10.  Pipette Stop Solution.

11.  Read color intensity at 450 nm.

12.  Evaluate the test.