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EIA EBV VCA IgM

EIA EBV VCA IgM
EAN Code: 8595635302787
Catalog number: VCM096
Package size: 96 tests
Producer: TestLine Clinical Diagnostics s.r.o.

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Contact person:

Name: Ing. Michal Šteffl
Phone: +420 549 121 259
E-mail: sales@testlinecd.com

Enzyme immunoassay for the detection of IgM antibodies to the capsular antigen of Epstein-Barr Virus in human serum or plasma.

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Contact person:

Name: Ing. Michal Šteffl
Phone: +420 549 121 259
E-mail: sales@testlinecd.com

Description

Description:

·         Microtitre wells are coated with the purified and inactivated VCA antigen.

·         If present, specific antibodies bind to the antigen. The complex is labelled with Conjugate and detected through a colour reaction with substrate (TMB-Complete).

·         The kit enables 96 tests (including controls and calibrators) within the microtitre plate made of colour-coded strips and breakable wells.

·         High sensitivity and specificity.

·         Total assay time: approximately 1.5 h.

·         CUT-OFF included.

·         Calibrators (5, 20, 80, 160 AU/ml) included.

·         Quantitative evaluation in AU/ml units.

·         Controls, Conjugate and substrate (TMB-Complete) are supplied in working strength.

·         Sample diluent, TMB-Complete and Avidity Solution are interchangeable if provided with identical numeric marking (i.e. Sample Diluent 2, Sample Diluent 3, etc.).  Stop Solution and Wash Solution are interchangeable in all TestLine kits.

·         Colour reagents for easy pipetting.

Applications:

·         Screening test for the detection of infection with EBV in humans.

·         Disease phase diagnostics.

Brief assay procedure:

·         Dilute samples (1:101).

·         Pipette controls and diluted samples.

·         Incubate for 30 min at 37 °C.

·         Aspirate and wash the wells 5 times.

·         Add Conjugate.

·         Incubate for 30 min at 37 °C.

·         Aspirate and wash the wells 5 times.

·         Add substrate (TMB-Complete).

·         Incubate for 30 min at 37 °C.

·         Add Stopping solution (H2SO4).

·         Read photometrically at 450 nm.

·         Evaluate results.

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Have a question? Need a help?

Ing. Michal Šteffl

 

Phone: +420 549 121 259

E-mail: sales@testlinecd.com