Description:

• Microtiter wells are coated with purified and inactivated HSV-2 antigen with a high content of specific immunodominant epitopes.
• If specific antibodies are present, they bind to the antigen, are labeled by the Conjugate in the following steps and are detected by color reaction with a single component substrate (TMB-Complete).
• The kit allows 96 tests, including controls in a split microtiter plate with color-coded strips and breakable wells.

• The total assay time is about 2 hours.
• High sensitivity and specificity of the test.
• Kit includes CUT-OFF, Positive Control and Negative Control, Calibrators.
• Semi-quantitative evaluation in the Index of Positivity (IP).
• Ready-to-use, color-coded components.
• Single-component substrate.
• Interchangeable components with the exception of kit specific components (Controls, Conjugate, Plate).

• Screening test for the detection of human Herpes simplex infection.
• To check the therapy results using the semiquantitative determination.

1. Dilute samples (1:101).
2. Pipette Controls and diluted Samples.
3. Incubate at 37°C for 30 min.
4. Aspirate and wash the wells 5×.
5. Pipette Conjugate.
6. Incubate at 37°C for 30 min.
7. Aspirate and wash the wells 5×.
8. Pipette Substrate (TMB-Complete).
9. Incubate at 37°C for 30 min.
10. Pipette Stop Solution.
11. Read color intensity at 450 nm
12. Evaluate the test.