Description:

• Microtiter wells are coated with purified and inactivated HSV-2 antigen with a high content of specific immunodominant epitopes.
• If present, specific antibodies bind to the antigen. The complex is labelled with Conjugate and detected through a color reaction with substrate (TMB-Complete).
• The kit enables 96 tests (including controls) within the microtiter plate made of color-coded strips and breakable wells.
• Total assay time: approximately 1.5 h.
• High sensitivity and specificity.
• CUT-OFF included.
• Semiquantitative evaluation using the index of positivity (IP).
• Controls, Conjugate and substrate (TMB-Complete) are supplied in working dilution.
• Sample diluent, TMB-Complete and Avidity Solution are interchangeable if provided with identical numeric marking (i.e. Sample Diluent 2, Sample Diluent 3, etc.). Stop Solution and Wash Solution are interchangeable in all TestLine kits.

• Searching test for detection of human infection Herpes simplex.
• Checking of therapy results using the semiquantitative determination.

1. Dilute samples (1:101)
2. Pipette controls and diluted samples
3. Incubate for 30 min at 37 °C
4. Aspirate and wash the wells 5 times
5. Add Conjugate
6. Incubate for 30 min at 37 °C
7. Aspirate and wash the wells 5 times
8. Add substrate (TMB-Complete)
9. Incubate for 30 min at 37 °C
10. Add Stopping solution (H2SO4)
11. Read photometrically at 450 nm
12. Evaluate results