Description:

·        A native antigen is bound to the well surface of the microtiter plate.

·        If specific antibodies are present, they bind to the antigen, labelled Conjugate in the following steps and are detected by color reaction with a single component substrate (TMB-Complete).

·        The kit allows 96 tests, including controls in a split microtiter plate with color-coded strips and breakable wells.

·        The total assay time is about 1.5 hours.

·        High sensitivity and specificity of the test.

·        Color solutions make work easier and reduce test error.

·        Possibility of gradual processing of the kit, the solutions are in sufficient excess.

·        Kit includes CUT-OFF.

·        Semi-quantitative evaluation in the Index of Positivity (IP).

·        Kit contains solutions in working dilution and single component substrate (TMB-Complete).

·        Sample Diluent, TMB-Complete and Avidity Solution are interchangeable in TestLine EIA kits if they have the same numerical indication. Stop and Wash Solution are universal.

Application:

·        Screening test for Influenza A virus infection.

Brief assay procedure:

1.      Dilute samples (1:101)

2.      Incubation at 37 °C for 10 min.

3.      Pipette Controls and diluted Samples

4.      Incubate at 37°C for 30 min.

5.      Aspirate and wash the wells 5×

6.      Pipette Conjugate

7.      Incubate at 37°C for 30 min.

8.      Aspirate and wash the wells 5×

9.      Pipette Substrate (TMB-Complete)

10.   Incubate at 37°C for 30 min.

11.   Pipette Stop Solution

12.   Read colour intensity at 450 nm

13.   Evaluate the test