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EIA Milk IgG

EIA Milk IgG
EAN Code: 8595635303197
Catalog number: MiG096
Package size: 96 tests
Regulatory status: IVD CE
Producer: TestLine Clinical Diagnostics s.r.o.

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Contact person:

Name: Ing. Zuzana Petříčková
Phone: +420 549 121 273
E-mail: petrickova@testlinecd.com

The immunoenzymatic assay is intended for the diagnosis of cow's milk intolerance using IgG antibodies in human serum or plasma in the general population. The semi-quantitative and quantitative manual assay is designed for professional use in a laboratory.

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Description

Description:

  • Microtitre wells are coated with the purified and native inactivated antigen.
  • If specific antibodies are present, they bind to the antigen, are labeled by the Conjugate in the following steps and are detected by color reaction with a single component substrate (TMB-Complete).
  • The kit allows 96 tests, including controls in a split microtiter plate with color-coded strips and breakable wells.

Advantages:

  • The total assay time is about 1 hour 30 min.
  • High sensitivity and specificity of the test.
  • Kit includes CUT-OFF, Positive Control, Negative Control and Calibrators (5, 20, 50, 100 U/ml).
  • Semi-quantitative evaluation in the Index of Positivity (IP) or quantitative evaluation in U/ml.
  • Ready-to-use, color-coded components.
  • Single-component substrate.
  • Interchangeable components with the exception of kit specific components (Controls, Conjugate, Plate).

Application:

  • Screening test for the detection of cow's milk intolerance in humans.
  • Monitoring of milk free diet effect.

Brief assay procedure:

  1. Dilute samples (1:101).
  2. Pipette Controls and diluted Samples.
  3. Incubate at 37°C for 30 min.
  4. Aspirate and wash the wells 5×
  5. Pipette Conjugate.
  6. Incubate at 37°C for 30 min.
  7. Aspirate and wash the wells 5×
  8. Pipette Substrate (TMB-Complete).
  9. Incubate at 37°C for 15 min.
  10. Pipette Stop Solution.
  11. Read color intensity at 450 nm.
  12. Evaluate the test.
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    Have a question?
    Need a help?

    Ing. Zuzana Petříčková

    +420 549 121 273 petrickova@testlinecd.com