Description:

·         Microtitre wells are coated with a chemically inactivated antigen specific for Tetanus Toxoid.

·         If specific antibodies are present, they bind to the antigen. The complex is labelled with Conjugate and detected through a colour reaction with substrate (TMB-Complete).

·         The kit enables 96 tests (including controls) within the microtitre plate made of colour-coded strips and breakable wells.

·         The total assay time is approximately 1.5 h.

·         High sensitivity and specificity of the test.

·         Quantitative evaluation of results (IU/ml).

·         Colour reagents for easy pipetting.

·         Calibrators, Conjugate, Sample Diluent and substrate (TMB-Complete) are supplied in working strength.

·         Sample Diluent, TMB-Complete are interchangeable in TestLine EIA kits if they have the same numerical indication. Stop and Wash Solution are universal.

Application:

·         Screening after vaccination

·         Detection of antibody level before booster dose to avoid potential hyperimmunity damage

Brief assay procedure:

1.       Dilute samples (1:101)

2.       Pipette Controls and diluted Samples

3.       Incubate at 37°C for 30 min.

4.       Aspirate and wash the wells 5×

5.       Pipette Conjugate

6.       Incubate at 37°C for 30 min.

7.       Aspirate and wash the wells 5×

8.       Pipette Substrate (TMB-Complete)

9.       Incubate at 37°C for 30 min.

10.   Pipette Stop Solution

11.   Read colour intensity at 450 nm

12.   Evaluate the test