Description:

• Microtitre wells are coated with a chemically inactivated antigen specific for Tetanus Toxoid.
• If specific antibodies are present, they bind to the antigen, are labeled by the Conjugate in the following steps and are detected by color reaction with a single component substrate (TMB-Complete).
• The kit allows 96 tests, including controls in a split microtiter plate with color-coded strips and breakable wells.

• The total assay time is about 2 hours.
• High sensitivity and specificity of the test.
• Kit includes CUT-OFF, Positive Control, Negative Control and Calibrators (0.1, 1, 2.5, 5 IU/ml).
• Semi-quantitative evaluation in the Index of Positivity (IP) or quantitative evaluation in IU/ml.
• Ready-to-use, color-coded components.
• Single-component substrate.
• Interchangeable components with the exception of kit specific components (Controls, Conjugate, Plate).

• Screening after vaccination.
• Detection of antibody level before booster dose to avoid potential hyper-immunity damage.

1. Dilute samples (1:101).
2. Pipette Controls and diluted Samples.
3. Incubate at 37°C for 30 min.
4. Aspirate and wash the wells 5×
5. Pipette Conjugate.
6. Incubate at 37°C for 30 min.
7. Aspirate and wash the wells 5×
8. Pipette Substrate (TMB-Complete).
9. Incubate at 37°C for 30 min.
10. Pipette Stop Solution.
11. Read color intensity at 450 nm.
12. Evaluate the test.