Menu Search My Account
Registration

Login

Registration Restore password
Cart Empty cart
cz en

EIA Toxocara IgG

EIA Toxocara IgG
EAN Code: 8595635303036
Catalog number: TcG096
Package size: 96 tests
Producer: TestLine Clinical Diagnostics s.r.o.

Sample request

Sample ordering is possible only for registered users

If you are already registered, please log in
If you are new customer register

Contact person:

Name: Ing. Michal Šteffl
Phone: +420 549 121 259
E-mail: sales@testlinecd.com

Enzyme immunoassay for the detection of IgG antibodies and their avidity to Toxocara sp.in human serum or plasma.

If you are already registered, please login

Request Product Export to PDF Print

Have a question? Need a help?

Do not hesitate and call us or fill in a query form and we will contact you soon.

Description

Description:

·         Microtitre wells are coated with the excretory-secretory ('ES') antigen isolated from the cultured larvae of Toxocara canis.

·         If present, specific antibodies bind to the antigen. The complex is labelled with Conjugate and detected through a colour reaction with substrate (TMB-Complete).

·         The kit enables 96 tests (including controls) within the microtitre plate made of colour-coded strips and breakable wells.

·         The kit contains the Avidity solution, which enables quantitative determination of bond strength of an antibody-antigen complex. Based on this fact, it is possible to distinguish between acute and chronic phase of the disease.

·         High sensitivity and specificity.

·         Total assay time: approximately 1.5 h.

·         CUT-OFF included.

·         Semiquantitative evaluation using the index of positivity (IP).

·         Controls, Conjugate and substrate (TMB-Complete) are supplied in working strength.

·         Colour reagents for easy pipetting.

·         Each batch is tested in the National Reference Laboratory.

·         Shelf life: 18 months.

Application:

·         Laboratory screening test for the detection of toxocariasis (larva migrans).

·         Differential diagnosis of acute and chronic phase of the disease.

·         Monitoring of antibody levels following therapy.

Brief assay procedure:

1.       Dilute samples (1:101).

2.       Pipette controls and diluted samples.

3.       Incubate for 30 min at 37 °C.

4.       Aspirate and wash the wells 5 times.

5.       Add Conjugate.

6.       Incubate for 30 min at 37 °C.

7.       Aspirate and wash the wells 5 times.

8.       Add substrate (TMB-Complete).

9.       Incubate for 15 min at 37 °C.

10.   Add Stopping solution (H2SO4).

11.   Read photometrically at 450 nm.

12.   Evaluate results.

 

Download

Have a question? Need a help?

Ing. Michal Šteffl

 

Phone: +420 549 121 259

E-mail: sales@testlinecd.com