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EIA Toxoplasma IgE

EIA Toxoplasma IgE
EAN Code: 8595635303050
Catalog number: TgE096
Package size: 96 tests
Regulatory status: IVD CE2265
Producer: TestLine Clinical Diagnostics s.r.o.

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Contact person:

Name: Mgr. Miroslav Herczeg
Phone: +420 549 121 273
E-mail: herczeg@testlinecd.com

The immunoenzymatic assay is intended for the diagnosis of Toxoplasma gondii infection using IgE antibodies in human serum or plasma in the general population. The semi-quantitative manual assay is designed for professional use in a laboratory.

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Description

Description:

  • Microtitre wells are coated with the antibody to human IgE.
  • All the IgE antibodies in the sample, including specific anti-T.gondii antibodies (if present), bind to the anti-human IgE antibody in quantities proportionate to their presence. The specific antibodies bound to the solid phase are subsequently labelled with the Tracer (T. gondii antigen + anti-T.gondii monoclonal antibody to the surface protein p-30 of T. gondii conjugated with horseradish peroxidase) and detected through a colour reaction with substrate (TMB-Complete).
  • The kit allows 96 tests, including controls in a split microtiter plate with color-coded strips and breakable wells.

Advantages:

  • The total assay time is about 2 hours 30 minutes.
  • High sensitivity and specificity of the test.
  • Kit includes CUT-OFF, Positive Control and Negative Control, Calibrators.
  • Semi-quantitative evaluation in the Index of Positivity (IP).
  • Ready-to-use, color-coded components.
  • Single-component substrate.
  • Interchangeable components with the exception of kit specific components (Controls, Conjugate, Plate).

Application:

  • Screening test for the detection of acute infection with Toxoplasma gondii in humans.
  • Checking of therapy results using the semiquantitative determination.

Brief assay procedure:

  1. Dilute samples (1:101).
  2. Pipette Controls and diluted Samples.
  3. Incubate at 37°C for 60 min.
  4. Aspirate and wash the wells 5×
  5. Pipette Conjugate.
  6. Incubate at 37°C for 60 min.
  7. Aspirate and wash the wells 5×
  8. Pipette Substrate (TMB-Complete).
  9. Incubate at 37°C for 20 min.
  10. Pipette Stop Solution.
  11. Read color intensity at 450 nm.
  12. Evaluate the test.
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Have a question?
Need a help?

Mgr. Miroslav Herczeg

+420 549 121 273 herczeg@testlinecd.com