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EIA Treponema pallidum IgG

EIA Treponema pallidum IgG
EAN Code: 8595635303081
Catalog number: TpG096
Package size: 96 tests
Producer: TestLine Clinical Diagnostics s.r.o.

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Contact person:

Name: Ing. Michal Šteffl
Phone: +420 549 121 259

Enzyme immunoassay for the detection of IgG antibodies to Treponema pallidum in human serum or plasma.

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·         Microtitre wells are coated with the specific antigens of Treponema pallidum, particularly p17, p47, p41 and p15.

·         If present, specific antibodies bind to the antigen. The complex is labelled with the Conjugate and detected through a colour reaction with substrate (TMB-Complete).

·         III. generation kit, high sensitivity and specificity.

·         The kit enables 96 tests (including controls) within the microtitre plate made of colour-coded strips and breakable wells.

·         Total assay time: approximately 2 h 15 min.

·         CUT-OFF included.

·         Semiquantitative evaluation using the index of positivity (IP).

·         Sample diluent, TMB-Complete and Avidity Solution are interchangeable if provided with identical numeric marking (i.e. Sample Diluent 2, Sample Diluent 3, etc.).  Stop Solution and Wash Solution are interchangeable in all TestLine kits.

·         Reagents are supplied in final working strength.

·         Colour reagents for easy pipetting.


·         Highly sensitive and specific ELISA method for detection of anti-treponema IgG is suitable for screening as well as confirmation of non-treponemal (VDRL, RPR, etc.) and treponemal (TPHA, etc.) test results.

·         Determination of IgG and IgM antibodies enables to distinguish between contemporary and previously undergone infection, diagnostics of congenital infection, antibiotic treatment efficiency monitoring.

Brief assay procedure:

1.       Dilute serum/plasma samples (1:51).

2.       Pipette controls and diluted samples.

3.       Incubate for 60 min at 37 °C.

4.       Aspirate and wash the wells 5 times.

5.       Add Conjugate.

6.       Incubate for 30 min at 37 °C.

7.       Aspirate and wash the wells 5 times.

8.       Add substrate (TMB-Complete).

9.       Incubate for 30 min at 37 °C.

10.   Add Stopping solution (H2SO4).

11.   Read photometrically at 450 nm.

12.   Evaluate results.


Have a question? Need a help?

Ing. Michal Šteffl


Phone: +420 549 121 259