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EIA Treponema pallidum TOTAL

EIA Treponema pallidum TOTAL
EAN Code: 8595635303104
Catalog number: Tp0096
Package size: 96 tests
Regulatory status: IVD CE
Producer: TestLine Clinical Diagnostics s.r.o.

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Contact person:

Name: Mgr. Miroslav Herczeg
Phone: +420 549 121 273
E-mail: herczeg@testlinecd.com

The immunoenzymatic assay is intended for the diagnosis and screening of Treponema pallidum infection using total antibodies in human serum or plasma in the general population. The semi-quantitative manual assay is designed for professional use in a laboratory.

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Description

Description:

  • Microtitre wells are coated with the specific antigens of strain Treponema pallidum, mainly p17, p47, p41, p15.
  • If specific antibodies are present, they bind to the antigen, are labeled by the Conjugate in the following steps and are detected by color reaction with a single component substrate (TMB-Complete).
  • The kit allows 96 tests, including controls in a split microtiter plate with color-coded strips and breakable wells.

Advantages:

  • The total assay time is about 1 hours 15 minutes.
  • High sensitivity and specificity of the test.
  • Kit includes CUT-OFF, Positive Control and Negative Control, Calibrators.
  • Semi-quantitative evaluation in the Index of Positivity (IP).
  • Ready-to-use, color-coded components.
  • Single-component substrate.
  • Interchangeable components with the exception of kit specific components (Controls, Conjugate, Plate).

Application:

  • Qualitative test for detection of antibodies against Treponema pallidum.

Brief assay procedure:

  1. Samples are used in undiluted form.
  2. Pipette controls and undiluted samples.
  3. Add Conjugate.
  4. Incubate for 30 min at 37 °C.
  5. Aspirate and wash the wells 5 times.
  6. Add substrate (TMB-Complete).
  7. Incubate for 30 min at 37 °C.
  8. Add Stopping solution (H2SO4).
  9. Read photometrically at 450 nm.
  10. Evaluate results.
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Have a question?
Need a help?

Mgr. Miroslav Herczeg

+420 549 121 273 herczeg@testlinecd.com