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EIA Treponema pallidum TOTAL

EIA Treponema pallidum TOTAL
EAN Code: 8595635303104
Catalog number: Tp0096
Package size: 96 tests
Regulatory status: IVD CE
Producer: TestLine Clinical Diagnostics s.r.o.

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Contact person:

Name: Mgr. Miroslav Herczeg
Phone: +420 549 121 273

The immunoenzymatic assay is intended for the diagnosis and screening of Treponema pallidum infection using total antibodies in human serum or plasma in the general population. The semi-quantitative manual assay is designed for professional use in a laboratory.

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  • Microtitre wells are coated with the specific antigens of strain Treponema pallidum, mainly p17, p47, p41, p15.
  • If specific antibodies are present, they bind to the antigen, are labeled by the Conjugate in the following steps and are detected by color reaction with a single component substrate (TMB-Complete).
  • The kit allows 96 tests, including controls in a split microtiter plate with color-coded strips and breakable wells.


  • The total assay time is about 1 hours 15 minutes.
  • High sensitivity and specificity of the test.
  • Kit includes CUT-OFF, Positive Control and Negative Control, Calibrators.
  • Semi-quantitative evaluation in the Index of Positivity (IP).
  • Ready-to-use, color-coded components.
  • Single-component substrate.
  • Interchangeable components with the exception of kit specific components (Controls, Conjugate, Plate).


  • Qualitative test for detection of antibodies against Treponema pallidum.

Brief assay procedure:

  1. Samples are used in undiluted form.
  2. Pipette controls and undiluted samples.
  3. Add Conjugate.
  4. Incubate for 30 min at 37 °C.
  5. Aspirate and wash the wells 5 times.
  6. Add substrate (TMB-Complete).
  7. Incubate for 30 min at 37 °C.
  8. Add Stopping solution (H2SO4).
  9. Read photometrically at 450 nm.
  10. Evaluate results.

Have a question?
Need a help?

Mgr. Miroslav Herczeg

+420 549 121 273