Menu Search My Account


Registration Restore password
Cart Empty cart
cz en


EAN Code: 8595635303128
Catalog number: IPN096
Package size: 96 tests
Producer: TestLine Clinical Diagnostics s.r.o.

Sample request

Sample ordering is possible only for registered users

If you are already registered, please log in
If you are new customer register

Contact person:

Name: MVDr. Ivo Hofmann, CSc.
Phone: +420 549 121 209

Enzyme immunoassay for the detection of Infectious Pancreatic Necrosis Virus (IPNV) antigen in salmonid fish organ homogenates and in culture media of infected cell cultures.

If you are already registered, please login

Request Product Export to PDF Print

Have a question? Need a help?

Do not hesitate and call us or fill in a query form and we will contact you soon.



·         Microtitre wells are coated with the specific antibodies to IPN virus.

·         The kit enables 96 screening tests, including controls.

·         The whole kit may be used at one time or sequentially for smaller batches of samples.

·         Assay sensitivity (102 TCID50/ 0.1 ml of the examined fluid) is sufficient for the routine examination of samples.

·         Total time of survey according to sample incubation duration: a) over night: results in second day. b) rapid test: approximately 2.5 h

·         Tested by the Veterinary Research Institute, Brno, Czech Republic and the State Veterinary Institutes in the Czech Republic.

·         Approved by the Institute for State Control of Veterinary Biologicals and Medicaments, Czech Republic.

·         The kit is manufactured in accordance with Good Manufacturing Practice (GMP).

·         Shelf life: 18 months.


·         Detection of IPNV in organ homogenates and in the media of infected cell cultures.

·         Diagnosis of the infection.

·         Control of fish before transport.

·         Control of quarantined fish (export, import).

Brief assay procedure:

1.       Dilute and pipette samples, control antigens and RAIPNV into the wells:

a) screening test: 1+1 (sample + RAIPNV) directly in the wells.

b) titration test: 1+1 (sample + RAIPNV) to 1+255, directly in the wells.

2.       Incubate:

a) over night: 14-18 hours at 4-8°C

b) rapid test: 60 min at 37°C

3.       Aspirate and wash the wells 4 times.

4.       Add Conjugate (an antibody to rabbit IgG labelled with peroxidase).

5.       Incubate for 60 min at 37 °C.

6.       Aspirate and wash the wells 4 times.

7.       Add substrate (TMB-Complete).

8.       Incubate for 10 min at room temperature (20 °C).

9.       Add Stopping solution (H2SO4).

10.   Read absorbance photometrically at 450 nm.

11.   Evaluate results.


Have a question? Need a help?

MVDr. Ivo Hofmann, CSc.


Phone: +420 549 121 209