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Leishmania infantum IgG ELISA

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Leishmania infantum IgG ELISA
Catalog number: LiG096 
Producer: NovaTec Immundiagnostica GmbH
Delivery time: 2-3 weeks
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Contact person:

Name: Mgr. Miroslav Herczeg
Phone: +420 549 121 273
E-mail: herczeg@testlinecd.com

The Leishmania infantum IgG ELISA is intended for the qualitative determination of IgG class antibodies against Leishmania infantum in human serum or plasma (citrate, heparin).

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Description

Description:

·         Microtitre wells are coated with specific antigens.

·         If specific antibodies are present, they bind to the antigen, in the following steps they are labelled with conjugate and are detected by color reaction with a single component substrate.

·         The kit allows 96 tests, including controls in a split microtiter plate with breakable wells.

·         The total assay time is about 2 hours.

·         High sensitivity and specificity of the test.

·         Kit includes CUT-OFF, evaluation using NTU units.

·         The kit contains ready-to-use reagents and a single component substrate.

·         Color-coded and interchangeable reagents for high user comfort.

·         Possibility of gradual processing of the kit, reagents are in sufficient quantity.

·         Kits supplied in cooperation with NovaTec Immundiagnostica GmbH.

Application:

·         Screening test for the evidence of human infection.

·         Distinguishing the stage of the disease.

Brief assay procedure:

1.       Dilute samples of serum/plasma (1:101).

2.       Pipette Controls and diluted Samples.

3.       Incubate at 37°C for 60 min.

4.       Aspirate and wash the wells 3×.

5.       Pipette Conjugate.

6.       Incubate at laboratory temperature for 30 min.

7.       Aspirate and wash the wells 3×.

8.       Pipette Substrate.

9.       Incubate at laboratory temperature for 15 min.

10.   Pipette Stop Solution.

11.   Read color intensity at 450 nm.

12.   Evaluate the test.

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Have a question?
Need a help?

Mgr. Miroslav Herczeg

+420 549 121 273 herczeg@testlinecd.com