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LEPTOSPIRA- Medium medicated

LEPTOSPIRA- Medium medicated
EAN Code: 8595635303449
Catalog number: LMm020
Package size: 20 ml

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Contact person:

Name: Mgr. Miroslav Herczeg
Phone: +420 549 121 273
E-mail: herczeg@testlinecd.com

Korthof's liquid germ-free medium designed to heal contaminated Leptospira cultures.

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Description

Product information:

  • The selective liquid medium is enriched with a mixture of 5-fluorouracil and rifampicin. The active substances act to remove contamination of innoculated leptospira strains.

 

Active substance

Effective

 Mostly ineffective

   Ineffective

5-fluorouracil

all yeasts of the genus

 

 

Candida, Torulopsis, Cryptococcus, Cladosporium
some molds e.g: Aspergillus

   

Rifampicin

Genus streptococcus

Enterococcus faecium

Pseudomonas spp.

Enterococcus faecalis

Enterobacter spp.

Acinetobacter spp.

Staphylococcus aureus

Pantoea spp.

 

most of the coagulase negative staphylococci

Serratia spp.

 

genus bacillus

   

genus micrococcus

   

most psychrophilic organisms
E. coli
Klebsiella spp.
genus Proteus

   

 

Brief assay procedure:

  • Transfer the contaminated culture with a Pasteur pipette to the medicated medium, dispense 300-500 µl and perform a microscopic examination after 48 hours. At this time, the bacterial infection should be suppressed, re-inoculate into the growth medium. For better growth, we recommend repeating the inoculation into the growth medium after 7 days.

Note:

  • Only cultures with a sufficient amount of leptospira (growth to at least ++) are suitable for healing. If the contamination is massive and there is not enough leptospira, the culture can only be cleared. However, strain growth remains small, unsuitable for MAL or re-innoculation.

Packaging:

  • 20 tubes with 3 ml of media each.

Storage and expiration:

  • Store in a dry place at a temperature of +2 to + 8 ° C. Subject to these conditions, the expiration date is 12 months from the date of manufacture
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Have a question?
Need a help?

Mgr. Miroslav Herczeg

+420 549 121 273 herczeg@testlinecd.com