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Microblot-Array EBV IgA

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Microblot-Array EBV IgA
EAN Code: 8595635308734
Catalog number: EBAMA96
Package size: 96 tests
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Contact person:

Name: Mgr. Miroslav Herczeg
Phone: +420 549 121 273
E-mail: herczeg@testlinecd.com

Microblot-Array kit is used for the detection of specific IgM antibodies against recombinant Epstein-Barr virus antigens in human serum or plasma

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Description

Description:

·         Used Antigens: combination of selected parts of the specific antigens of EBV (EBNA-1, EBNA-2, VCA p18, VCA p23, EA-D p54, EA-D p138, EA-R, Rta, ZEBRA, gp85, gp350 and LMP1).

·         Recombinant antigens are applied in the form of microdots (spots) in triplets to a nitrocellulose membrane in a microtiter well format.

·         If specific antibodies are present in the sample, they will bind to the respective antigens.

·         The complex is labelled with Conjugate and detected through a colour reaction with substrate (BCIP/NBT).

·         The kit enables 96 tests.

·         Short incubation periods, total assay time: approximately 1.5 h.

·         Each individual test includes control spots, which serves to check functionality and sensitivity of the sets, and also the spots for checking the presence of the conjugate.

·         Positive controls included enabling validation of the tests.

·         For quantitative evaluation the wells contain calibration spots.

·         All reagents supplied are ready to use.

·         The kit may be used sequentially for smaller batches of samples (reagents are provided in sufficient quantities).

·         Evaluation using Microblot-Array reader and the appropriate software.

Applications:

  • Laboratory test for the detection of EBV
  • Confirmatory test to ELISA.

Brief assay procedure:

1.      Dilute samples serum/plasma (1:51).

2.      Pipette diluted samples.

3.      Incubate at room temperature for 30 min.

4.      Wash 3 times for 5 min.

5.      Add Conjugate.

6.      Incubate at room temperature for 30 min.

7.      Wash 3 times for 5 min.

8.      Add Substrate (BCIP/NPT).

9.      Incubate at room temperature for 15 min.

10.  Wash 2 times for 5 min in distilled water.

11. Dry and evaluate of test.

 

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Have a question?
Need a help?

Mgr. Miroslav Herczeg

+420 549 121 273 herczeg@testlinecd.com

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