Description:

·         The kit allows 480 screening (single-well) examinations, including controls.

·         The kit can be used once or smaller sample sets can processed sequentially.

·         Purified and inactivated M. avium spp. Paratuberculosis is bound in microtiter wells.

·         Total examination time is about 2.5 hours.

·         Evaluation method: ratio of the absorbance of the sample to the absorbance of the positive control limit serum expressed as a percentage (%).

·         The kit is verified at the Veterinary Research Institute in Brno and the State Veterinary Institutes.

·         The kit is approved by the Institute for State Control of Veterinary Biologicals and Medicines.

·         The kit is manufactured in accordance with Good Laboratory Practice (GMP).

·         Expiration of the kit is 12 months from the date of manufacture.

Application:

·         Screening for specific IgG antibodies in bovine serum, plasma or milk.

·         Differential diagnostics of diarrhea.

·         Prevention and control programs for the recovery of cattle.

Brief assay procedure:

1.       Dilution of serum samples and control sera

-          Serum/Plasma/Control Sera: 1:12 (10 μl + 110 μl)

-          Milk: 1:2 (60 μl + 60 μl)

2.       Incubate at 21°C for 5- 45 min

3.       Dispense of diluted control sera and samples: 100 μl

4.       Incubate at 21°C for 30 min

5.       Aspirate and wash the wells 4×

6.       Pipette Conjugate – 100 μl

7.       Incubate at 21°C for30 min

8.       Aspirate and wash the wells 4×

9.       Pipette Substrate (TMB-Complete) – 100 μl

10.   Incubate at room temperature for 15 min

11.   Pipette Stop Solution – 100 μl

12.   Read colour intensity at 450 nm