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EAN Code: 8595635306570
Catalog number: SK-ScA096
Package size: 96 tests
Producer: TestLine Clinical Diagnostics s.r.o.

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Contact person:

Name: Ing. Michal Šteffl
Phone: +420 549 121 259

Enzyme immunoassays for the detection of IgA antibodies to Saccharomyces cerevisiae (ASCA) in human serum or plasma. SmartEIA kit is specifically designed for automated analysis using the Agility instrument.

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·         Microtitre wells are coated with high purified antigen mannan isolated from Saccharomyces cerevisiae.

·         If present, specific antibodies bind to the antigen. The complex is labelled with Conjugate and detected through a colour reaction with substrate (TMB-Complete).

·         The kit enables 96 tests (including controls and calibrators) within the microtitre plate made of colour-coded strips and breakable wells.

·         Calibrators included (5, 20, 80, 320 AU/ml).

·         Semiquantitative evaluation using the index of positivity (IP) or quantitative evaluation in AU/ml units.

·         Total assay time: approximately 1 h and 15 min.

·         High sensitivity and specificity.

·         Colour reagents for easy pipetting.


·         Laboratory test for differential diagnostics of inflammatory bowel disease (IBD).

Brief assay procedure:

1.       Dilute samples (1:51).

2.       Pipette controls, calibrators and diluted samples.

3.       Incubate for 30 min at 37 °C.

4.       Aspirate and wash the wells 5 times.

5.       Add Conjugate.

6.       Incubate for 15 min at 37 °C.

7.       Aspirate and wash the wells 5 times.

8.       Add substrate (TMB-Complete).

9.       Incubate 15 min at 37 °C.

10.   Add Stopping solution (H2SO4).

11.   Read photometrically at 450 nm.

12.   Evaluate results.


Have a question? Need a help?

Ing. Michal Šteffl


Phone: +420 549 121 259