·         Microtitre wells are coated with the sonicated whole-cell antigen of the Borrelia afzelii KC90 strain rich in OspA, OspC, p41 and p100.

·         If specific antibodies are present, they bind to the antigen, are labeled by the Conjugate in the following steps and are detected by color reaction with a single component substrate (TMB-Complete).

·         The kit allows 96 tests, including controls in a split microtiter plate with color-coded strips and breakable wells.

Advantages:

·         The total assay time is about 1 hour 30 min.

·         High sensitivity and specificity of the test.

·         Kit includes CUT-OFF, Positive Control and Negative Control.

·         Semi-quantitative evaluation in the Index of Positivity (IP).

·         Ready-to-use, color-coded components.

·         Single-component substrate.

·         Interchangeable components with the exception of kit specific components (Controls, Conjugate, Plate).

Application:

·         Screening test for the detection of Lyme borreliosis in humans.

·         Checking of therapy results using the semiquantitative determination.

Brief assay procedure:

1.       Dilute samples of serum/plasma (1:101), synovial fluid (1:21, 1:41) or cerebrospinal fluid (1:2).

2.       Pipette Controls and diluted Samples.

3.       Incubate at 37°C for 30 min.

4.       Aspirate and wash the wells 5×

5.       Pipette Conjugate.

6.       Incubate at 37°C for 30 min.

7.       Aspirate and wash the wells 5×

8.       Pipette Substrate (TMB-Complete).

9.       Incubate at 37°C for 15 min.

10.   Pipette Stop Solution.

11.   Read color intensity at 450 nm.