Description:

·         Microtitre wells are coated with the sonicated whole-cell antigen of Borrelia garinii rich in p83, p39, OspA, OspC, p41, p18 and p14.

·         If present, specific antibodies bind to the antigen. The complex is labelled with Conjugate and detected through a colour reaction with substrate (TMB-Complete).

·         The kit enables 192 tests (including controls) within the microtitre plate made of colour-coded strips and breakable wells.

·         Total assay time: approximately 1.5 h.

·         High sensitivity and specificity.

·         Controls, Conjugate and substrate (TMB-Complete) are supplied in working strength.

·         Sample diluent, TMB-Complete and Avidity Solution are interchangeable if provided with identical numeric marking (i.e. Sample Diluent 2, Sample Diluent 3, etc.).  Stop Solution and Wash Solution are interchangeable in all TestLine kits.

·         CUT-OFF included.

·         Colour reagents for easy pipetting.

·         Semiquantitative evaluation using the index of positivity (IP).

·         Detection of intrathecal production of specific antibodies with a help of the Borrelia garinii AI - Standard IgG and with Antibody Index Software (made by TEST-LINE).

Applications:

·         Screening test for the detection of Lyme borreliosis in humans.

·         Checking of therapy results using the semiquantitative determination.

·         Improvement in diagnostic of neuroborreliosis by the detection of intrathecal production of specific antibodies to Borrelia sp.

Brief assay procedure:

1.       Dilute samples of serum/plasma (1:101), synovial fluid (1:21, 1:41) or cerebrospinal fluid (1:2).

2.       Pipette controls and diluted samples.

3.       Incubate for 30 min at 37 °C.

4.       Aspirate and wash the wells 4 times.

5.       Add Conjugate.

6.       Incubate for 30 min at 37 °C.

7.       Aspirate and wash the wells 5 times.

8.       Add substrate (TMB-Complete).

9.       Incubate for 15 min at 37 °C.

10.   Add Stopping solution (H2SO4).

11.   Read photometrically at 450 nm.

12.   Evaluate results.