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SmartEIA COVID-19 RBD IgA

SmartEIA COVID-19 RBD IgA
EAN Code: 8595635309274
Catalog number: SK-CoRA96
Package size: 96 tests
Regulatory status: IVD CE

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Contact person:

Name: Mgr. Miroslav Herczeg
Phone: +420 549 121 273
E-mail: herczeg@testlinecd.com

Immunoenzymatic kit for the determination of IgA antibodies against SARS-CoV-2 (COVID-19) in human serum or plasma. SmartEIA kit is specifically designed for automated analysis using the Agility instrument.

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Description

 Description:

  • Recombinant antigen Receptor-binding domain (RBD), a subunit of the Spike S1 protein, is bound to the well surface of the microtiter plate.
  • If specific antibodies are present, they bind to the antigen, are labeled by the Conjugate in the following steps and are detected by color reaction with a single component substrate (TMB-Complete).
  • The kit allows 96 tests, including controls in a split microtiter plate with color-coded strips and breakable wells.

Advantages:

  • The total assay time is about 1 hour 30 min.
  • High sensitivity and specificity of the test.
  • Kit includes CUT-OFF, Positive Control, Negative Control and Calibrators (5, 20, 80, 320, 640, 1000 BAU/ml).
  • Semi-quantitative evaluation in the Index of Positivity (IP) or quantitative evaluation in BAU/ml.
  • Ready-to-use, color-coded components.
  • Single-component substrate.
  • Interchangeable components with the exception of kit specific components (Controls, Conjugate, Plate).

Application:

  • Identification of patients with asymptomatic infection in the past.
  • Detection of naturally acquired and vaccination antibodies.
  • Prevalence studies.
  • Indication of neutralizing immunity.

Brief assay procedure:

  1. Dilute samples (1:101).
  2. Pipette Controls and diluted Samples.
  3. Incubate at 37°C for 30 min.
  4. Aspirate and wash the wells 5×
  5. Pipette Conjugate.
  6. Incubate at 37°C for 30 min.
  7. Aspirate and wash the wells 5×
  8. Pipette Substrate (TMB-Complete).
  9. Incubate at 37°C for 15 min.
  10. Pipette Stop Solution.
  11. Read color intensity at 450 nm.
  12. Evaluate the test.
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Have a question?
Need a help?

Mgr. Miroslav Herczeg

+420 549 121 273 herczeg@testlinecd.com