Description:
- Microtitre wells are coated with the purified and native inactivated antigen.
- If present, specific antibodies bind to the antigen. The complex is labelled with Conjugate and detected through a color reaction with substrate (TMB-Complete).
- The kit enables 96 quantitative tests (including controls and calibrators) within the microtitre plate made of color-coded strips and breakable wells.
- Total assay time: approximately 1.5 h.
- High sensitivity and specificity.
- Calibrators (5, 20, 80, 320 AU/ml) included.
- Quantitative evaluation in AU/ml units.
- Sample diluent, TMB-Complete and Avidity Solution are interchangeable if provided with identical numeric marking (i.e. Sample Diluent 2, Sample Diluent 3, etc.). Stop Solution and Wash Solution are interchangeable in all TestLine kits.
- Controls, Conjugate and substrate (TMB-Complete) are supplied in working strength.
- Color reagents for easy pipetting.
Applications:
- Diagnostics of Mumps.
- Checking of effectiveness of vaccination.
Brief assay procedure:
- Dilute controls and tested samples (1:101).
- Pipette diluted controls and samples.
- Incubate for 30 min at 37 °C.
- Aspirate and wash the wells 5 times.
- Add Conjugate.
- Incubate for 30 min at 37 °C.
- Aspirate and wash the wells 5 times.
- Add substrate (TMB-Complete).
- Incubate for 30 min at 37 °C.
- Add Stopping solution (H2SO4).
- Read photometrically at 450 nm.
- Evaluate results.
Ing. Michal Šteffl