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SmartEIA Parainfluenza mix IgG

SmartEIA Parainfluenza mix IgG
EAN Code: 8595635308482
Catalog number: SK-PaiG96
Package size: 96 tests

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Contact person:

Name: Mgr. Miroslav Herczeg
Phone: +420 549 121 273
E-mail: herczeg@testlinecd.com

Immunoenzymatic kit for the determination of IgG antibodies against Parainfluenza virus type 1, 2, 3 in human serum or plasma. SmartEIA kit is specifically designed for automated analysis using the Agility instrument.

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Description

Description:

·         A combination of antigens is bound to the well surface of the microtiter plate.

·         If specific antibodies are present, they bind to the antigen, labelled Conjugate in the following steps and are detected by colour reaction with a single component substrate (TMB-Complete).

·         The kit allows 96 tests, including controls in a split microtiter plate with color-coded strips and breakable wells.

·         The total assay time is about 1.5 hours.

·         Kit includes CUT-OFF.

·         High sensitivity and specificity of the test.

·         Colour solutions make work easier and reduce test error.

·         Possibility of gradual processing of the kit, the solutions are in sufficient excess.

·         Kit contains solutions in working dilution and single component substrate (TMB-Complete).

·         Sample Diluent, TMB-Complete and Avidity Solution are interchangeable in TestLine EIA kits if they have the same numerical indication. Stop and Wash Solution are universal.

·         Semiquantitative evaluation using the index of positivity (IP).

 

Application:

·         Screening test for the detection of infection caused by Parainfluenza virus type 1, 2, 3.

 

Brief assay procedure:

1.       Dilute samples (1:101)

2.       Pipette Controls and diluted Samples

3.       Incubate at 37°C for 30 min

4.       Aspirate and wash the wells 5×

5.       Pipette Conjugate

6.       Incubate at 37°C for 30 min

7.       Aspirate and wash the wells 5×

8.       Pipette Substrate (TMB-Complete)

9.       Incubate at 37°C for 30 min

10.   Pipette Stop Solution

11.   Read colour intensity at 450 nm

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Have a question?
Need a help?

Mgr. Miroslav Herczeg

+420 549 121 273 herczeg@testlinecd.com

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