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SmartEIA Tetanus Toxoid IgG

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SmartEIA Tetanus Toxoid IgG
EAN Code: 8595635309403
Catalog number: SK-TeTG96
Package size: 96 tests
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Contact person:

Name: Mgr. Miroslav Herczeg
Phone: +420 549 121 273
E-mail: herczeg@testlinecd.com

Enzyme immunoassays for the diagnostics of tetanus disease caused by the toxin produced by Clostridium tetani. SmartEIA kit is specifically designed for automated analysis using the Agility® instrument.

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Description

Description:

·         Microtitre wells are coated with a chemically inactivated antigen specific for Tetanus Toxoid.

·         If specific antibodies are present, they bind to the antigen. The complex is labelled with Conjugate and detected through a colour reaction with substrate (TMB-Complete).

·         The kit enables 96 tests (including controls) within the microtitre plate made of colour-coded strips and breakable wells.

·         The total assay time is approximately 1.5 h.

·         High sensitivity and specificity of the test.

·         Quantitative evaluation of results (IU/ml).

·         Colour reagents for easy pipetting.

·         Calibrators, Conjugate, Sample Diluent and substrate (TMB-Complete) are supplied in working strength.

·         Sample Diluent, TMB-Complete are interchangeable in TestLine EIA kits if they have the same numerical indication. Stop and Wash Solution are universal.

Application:

·         Screening after vaccination.

·         Detection of antibody level before booster dose to avoid potential hyperimmunity damage.

Brief assay procedure:

1.       Dilute samples (1:101)

2.       Pipette Controls and diluted Samples

3.       Incubate at 37°C for 30 min.

4.       Aspirate and wash the wells 5×

5.       Pipette Conjugate

6.       Incubate at 37°C for 30 min.

7.       Aspirate and wash the wells 5×

8.       Pipette Substrate (TMB-Complete)

9.       Incubate at 37°C for 30 min.

10.   Pipette Stop Solution

11.   Read colour intensity at 450 nm

12.   Evaluate the test

 

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Have a question?
Need a help?

Mgr. Miroslav Herczeg

+420 549 121 273 herczeg@testlinecd.com

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