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SmartEIA Treponema pallidum TOTAL

SmartEIA Treponema pallidum TOTAL
EAN Code: 8595635306501
Catalog number: SK-Tp0096
Package size: 96 tests
Producer: TestLine Clinical Diagnostics s.r.o.

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Contact person:

Name: Ing. Michal Šteffl
Phone: +420 549 121 259
E-mail: sales@testlinecd.com

Enzyme immunoassay for the detection of total antibodies against Treponema pallidum in human serum or plasma. SmartEIA kit is specifically designed for automated analysis using the Agility instrument.

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Description

Description:

·         Microtitre wells are coated with the specific antigens of strain Treponema pallidum, mainly p17, p47, p41, p15.

·         If present, specific antibodies bind to the antigen. The complex is labelled with Conjugate and detected through a colour reaction with substrate (TMB-Complete).

·         The kit enables 96 tests (including controls) within the microtitre plate made of colour-coded strips and breakable wells.

·         Total assay time: approximately 1 h 15 min.

·         High sensitivity and specificity.

·         Colour reagents for easy pipetting.

·         The kit may be used sequentially for smaller batches of samples, reagents are provided in sufficient quantities.

·         Semiquantitative evaluation using the index of positivity (IP).

·         Controls, Conjugate and substrate (TMB-Complete) are supplied in working strength.

·         Sample diluent, TMB-Complete and Avidity Solution are interchangeable if provided with identical numeric marking (i.e. Sample Diluent 2, Sample Diluent 3, etc.).  Stop Solution and Wash Solution are interchangeable in all TestLine kits.

·         Shelf life: 9 months.

Applications:

·         Qualitative test for detection of antibodies against Treponema pallidum.

Brief assay procedure:

1.       Samples are used in undiluted form.

2.       Pipette controls and undiluted samples.

3.       Add Conjugate.

4.       Incubate for 30 min at 37 °C.

5.       Aspirate and wash the wells 5 times.

6.       Add substrate (TMB-Complete).

7.       Incubate for 30 min at 37 °C.

8.       Add Stopping solution (H2SO4).

9.       Read photometrically at 450 nm.

10.   Evaluate results.

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Have a question? Need a help?

Ing. Michal Šteffl

 

Phone: +420 549 121 259

E-mail: sales@testlinecd.com