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SmartEIA Yersinia IgM

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SmartEIA Yersinia IgM
EAN Code: 8595635309496
Catalog number: SK-YeM096
Package size: 96 tests
Regulatory status: IVD CE
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Contact person:

Name: Mgr. Miroslav Herczeg
Phone: +420 549 121 273
E-mail: herczeg@testlinecd.com

Enzyme immunoassay for the detection of IgM antibodies to Yersinia in human serum or plasma. SmartEIA kit is specifically designed for automated analysis using the Agility instrument.

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Description

Description:

  • A mixture of highly specific recombinant Yersinia enterocolitica antigens and recombinant Yersinia pseudotuberculosis antigens is bound to the well surface of the microtiter plate.
  • If specific antibodies are present, they bind to the antigen, are labeled by the Conjugate in the following steps and are detected by color reaction with a single component substrate (TMB-Complete).
  • The kit allows 96 tests, including controls in a split microtiter plate with color-coded strips and breakable wells.

Advantages:

  • The total assay time is about 2 hours.
  • High sensitivity and specificity of the test.
  • Kit includes CUT-OFF, Positive Control and Negative Control, Calibrators.
  • Semi-quantitative evaluation in the Index of Positivity (IP).
  • Ready-to-use, color-coded components.
  • Single-component substrate.
  • Interchangeable components with the exception of kit specific components (Controls, Conjugate, Plate).

Application:

  • Detection of specific antibodies against Yersinia enterocolitica and Yersinia pseudotubeculosis.
  • Confirmation of post-infectious antibodies.
  • Diagnostics of the disease phase by a significant increase or decrease of antibodies.

Brief assay procedure:

  1. Dilute samples (1:101).
  2. Pipette Controls and diluted Samples.
  3. Incubate at 37°C for 30 min.
  4. Aspirate and wash the wells 5×
  5. Pipette Conjugate.
  6. Incubate at 37°C for 30 min.
  7. Aspirate and wash the wells 5×
  8. Pipette Substrate (TMB-Complete).
  9. Incubate at 37°C for 30 min.
  10. Pipette Stop Solution.
  11. Read color intensity at 450 nm.
  12. Evaluate the test.
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Have a question?
Need a help?

Mgr. Miroslav Herczeg

+420 549 121 273 herczeg@testlinecd.com